Establishment of screening system for the candidate compounds for anti-cancer drugs with iPS cell

利用iPS细胞建立抗癌药物候选化合物筛选体系

• 批准号: 23650587
• 负责人: FUKUDA Tomokazu
• 金额: $ 2.41万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23650587/
• 关键词: エピジェネティクス; 人工多能性幹細胞; ゲノム; サイレンシング; 脱メチル化

We focused on the common biological feature between cancer and iPS cell, and tried to establish the screening system for de-methylating agents for the genome. We introduced reprogramming four factors into E6/E7 immortalized mouse embryonic fibroblasts (MEF). The infected MEF showed iPS transformation. We successfully established mouse iPS cells, and found that reported activity is dramatically dropped down. We are trying to screen the mow molecular compounds, which causes the re-activation of the promoter activity.

我们着眼于癌症和iPS细胞之间的共同生物学特征，试图建立基因组去甲基化剂的筛选系统。我们在E6/E7永生化小鼠胚胎成纤维细胞（MEF）中引入了四个重编程因子。感染的MEF呈现iPS转化。我们成功地建立了小鼠iPS细胞，并发现报道的活性急剧下降。我们正在尝试筛选导致启动子活性重新激活的新分子化合物。

项目成果

Establishment of Induced Pluripotent Stem Cell Lines to Monitor the Silencing Status of Genomes

建立诱导多能干细胞系以监测基因组沉默状态

• 发表时间: 2013
• 期刊: Analytical Biochemistry
• 影响因子: 2.9
• 作者: Kenichiro Donai;Kengo Kuroda;Yijie Guo;Kyoung-Ha So;Hideko Sone;Masayuki Kobayashi;Katsuhiko Nishimori;Tomokazu Fukuda
• 通讯作者: Tomokazu Fukuda

Low-molecular-weight inhibitors of cell differentiation enable efficient growth of mouse iPS cells under feeder-free conditions

低分子量细胞分化抑制剂使小鼠 iPS 细胞在无饲养层条件下高效生长

• DOI: 10.1007/s10616-013-9686-8
• 发表时间: 2014
• 期刊: Cytotechnology
• 影响因子: 2.2
• 作者: Donai K;Inagaki A;So KH;Kuroda K;Sone H;Kobayashi M;Nishimori K;Fukuda T
• 通讯作者: Fukuda T

Establishment of induced pluripotent stem cell lines that allow monitoring of the re-programming status of genomes

建立诱导多能干细胞系，以监测基因组的重编程状态

• 发表时间: 2012
• 作者: Donai K.;Kuroda K.;So KH.;Inagaki A.;Nishimori K.;Fukuda T
• 通讯作者: Fukuda T

Antiproliferative effect of cathelicidin family peptide LL-37 analogue on colon cancer HCT116 p53+/+ and p53-/- cells.

Cathelicidin 家族肽 LL-37 类似物对结肠癌 HCT116 p53 / 和 p53-/- 细胞的抗增殖作用。

• 发表时间: 2012
• 期刊: Oncology Rep
• 作者: Kuroda;K.;Fukuda;T.;Yoneyama;H.;Katayama;M.;Isogai;H.;Okumura;K.;and Isogai;M
• 通讯作者: M

Establishment of a Reporter System to Monitor Silencing Status in induced Pluripotent Stem Cell Lines

建立监测诱导多能干细胞系沉默状态的报告系统

• DOI: 10.1016/j.ab.2013.08.014
• 发表时间: 2013
• 期刊: Anal Biochem
• 影响因子: 2.9
• 作者: Donai K;Kuroda K;Guo Y;So K-H;Sone H;Kobayashi M;Nishimori K and Fukuda T
• 通讯作者: Nishimori K and Fukuda T