Establishment of screening system for the candidate compounds for anti-cancer drugs with iPS cell

本研究针对肿瘤与iPS细胞的共同生物学特性，尝试建立基因组去甲基化剂的筛选体系。 我们将重编程因子导入E6/E7永生化小鼠胚胎成纤维细胞（MEF）。 感染的MEF表现出iPS转化。 我们成功地建立了小鼠iPS细胞，并发现报道的活性显着下降。 我们正试图筛选出能使启动子活性重新激活的分子化合物。

We focused on the common biological feature between cancer and iPS cell, and tried to establish the screening system for de-methylating agents for the genome. We introduced reprogramming four factors into E6/E7 immortalized mouse embryonic fibroblasts (MEF). The infected MEF showed iPS transformation. We successfully established mouse iPS cells, and found that reported activity is dramatically dropped down. We are trying to screen the mow molecular compounds, which causes the re-activation of the promoter activity.