Mechanizme analysis of accumulation of PFOS in blood

PFOS在血液中蓄积的机理分析

• 批准号: 23651053
• 负责人: OSHIMA Yuji
• 金额: $ 2.5万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23651053/
• 关键词: PFOS 結合タンパク質; 魚類; 血液; PFOS; 結合タンパク質

Perfluorooctane sulfonate (PFOS) was known to accumulate in blood of fish, bird and mammals. PFOS might bind with unknown substance in blood, however the mechanism is unclear. Thus, we performed to purify a PFOS binding substance from fish blood and confirmed its affinity to PFOS. As results, PFOS binding protein was successfully purified from the plasma of PFOS administered-puffer fish (Takifugu rubripes) from the 65-70% ammonium sulfate precipitation. N-terminal analysis strongly suggested that PFOS-binding protein was an apolipoprotein A-I in T. rubripes. In accorded this, PFOS was confirmed to bind with the 65-70% ammonium sulfate fraction prepared from non-PFOS treated T. rubripes. Those results indicated that PFOS was bind to apolipoprotein A-I. Thus, this may suggest disorder of physiological function because apolipoprotein A-I may play an important role in lipid metabolism in fish.

已知全氟辛烷磺酸会在鱼类、鸟类和哺乳动物的血液中积累。PFOS可能与血液中的未知物质结合，但其作用机制尚不清楚。因此，我们从鱼血液中纯化PFOS结合物质，并确认其与PFOS的亲和力。结果表明，经65-70%硫酸铵沉淀，从施用全氟辛烷磺酸的红鳍东方鲀（Takifugu rubripes）血浆中成功纯化出全氟辛烷磺酸结合蛋白。N-末端分析结果表明，该蛋白是一种载脂蛋白A-I。红鳍据此，证实全氟辛烷磺酸与未经全氟辛烷磺酸处理的T.红鳍这些结果表明PFOS与载脂蛋白A-I结合。因此，这可能表明生理功能障碍，因为载脂蛋白A-I可能在鱼类的脂质代谢中起重要作用。

项目成果

魚類におけるPFOSの体内動態及び血液蓄積機構の解明

阐明全氟辛烷磺酸在鱼类体内的药代动力学和血液蓄积机制

• 发表时间: 2013
• 作者: 高田穣;冨田純也;板谷(内田)亜希子;茂地智子;海野純也;前原善彦;石合正道;牟田朱美・本田匡人・島崎洋平・大嶋雄治
• 通讯作者: 牟田朱美・本田匡人・島崎洋平・大嶋雄治