A study on mechanism of tetrodotoxin poisoning in tiger pufferfish by binding protein

结合蛋白研究河鲀毒素中毒机制

• 批准号: 23380125
• 负责人: OSHIMA Yuji
• 金额: $ 11.98万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23380125/
• 关键词: 天然毒; フグ毒; トリブチルスズ; 結合タンパク質; フグ毒結合タンパク質; 遺伝子組み換え; トランスジェニック; 遺伝子重複; 遺伝子導入; テトロドトキシン; トランスジェニックメダカ; テトロドトキシン毒結合タンパク質; 結合性; トリブチルスズ結合タンパク質; 分子系統樹; 分子進化

In this study, we performed to elucidate the mechanism of tetrodotoxin (TTX) poisoning in tiger pufferfish. First, recombinants of TTX binding protein and tributyltin (TBT) binding protein were successfully obtained from Bombyx mori. The results of binding assay using the recombinant proteins showed that these proteins could bind with TTX or TBT suggesting their detoxification function. In addition, transgenic medaka strains which over expressed TTX or TBT binding protein were successfully established. The results of TBT injection test to the transgenic medaka embryo suggested its detoxification function. Molecular phylogenic study of toxin binding protein among several puffer fish species demonstrated a evolution pathway from TBT binding protein to TTX binding protein.

本研究旨在阐明河豚毒素(TTX)中毒的机制。首先，从家蚕中成功获得TTX结合蛋白和三丁基锡(TBT)结合蛋白的重组体。结合实验结果表明，这些重组蛋白均能与TTX或TBT结合，提示其具有解毒功能。此外，还成功地建立了高表达TTX或TBT结合蛋白的青竹转基因菌株。对转基因青竹胚胎的TBT注射试验结果表明其具有解毒作用。几种河豚毒素结合蛋白的分子系统发育研究证实了TBT结合蛋白向TTX结合蛋白的进化途径。

项目成果

Expression and functional characterization of recombinant tributyltin-binding protein type 2.

• DOI: 10.2131/jts.38.885
• 发表时间: 2013-12
• 期刊: The Journal of toxicological sciences
• 作者: Hina Satone;Eriko Akahoshi;A. Nakamura;J. M. Lee;Masato Honda;Y. Shimasaki;S. Kawabata;T. Kusakabe;Y. Oshima

Characterization and evolutionary analysis of tributyltin-binding protein (TBT-bp) and pufferfish saxitoxin and tetrodotoxin binding protein (PSTBP) genes in toxic and non-toxic pufferfishes

有毒和无毒河豚中三丁基锡结合蛋白（TBT-bp）以及河豚毒素和河豚毒素结合蛋白（PSTBP）基因的特征和进化分析

• 作者: Hashiguchi Y;Lee JM;Shiraishi M;Komatsu S;Miki S;Shimasaki Y;Mochioka N;Kusakabe T;Oshima Y
• 通讯作者: Oshima Y

Monitoring of tributyltin contamination of demersal fish in coastal water in East China Sea

东海近海底层鱼类三丁基锡污染监测

• 发表时间: 2014
• 期刊: J. Fac. Agr, Kyu. Univ
• 作者: Undap S;Honda M;Rumampuk N;Inoue S;Shimasaki Y;Rompas R;Mochioka N;Oshima Y
• 通讯作者: Oshima Y

Toxicity of tributyltin to tropical marine fish, Pterapogon kauderni, and its accumulation

三丁基锡对热带海洋鱼翼手龙的毒性及其积累

• 发表时间: 2013
• 期刊: Report of the center of Advanced Instrumental Analysis Kyushu University
• 作者: Undap S;Pelle W;Isono R;Kishida C;Honda M;Shimasaki Y;Oshima Y
• 通讯作者: Oshima Y

トリブチルスズやフグ毒と結合する魚類リポカリンタンパク質

与三丁基锡和河豚毒素结合的鱼脂质运载蛋白

• 发表时间: 2012
• 作者: 高瀬麻以;村田政隆;日比香子;任 恵峰;遠藤英明;Kabeya N;大嶋雄治
• 通讯作者: 大嶋雄治