Population structure of pilchards using sequence of mitochondrial DNA

本研究旨在阐明日本沿海地区沙丁鱼的种群结构。方法从日本沿海地区采集沙丁鱼（Sardinops melanostictus）、圆斑鳀（Engraulis terus）和圆斑石斑鱼（Etrumeus teres）肌肉组织，提取总DNA。用酚-氯仿法从乙醇保存的肌肉标本中提取DNA。采用聚合酶链反应（PCR）技术，从侏儒鸟总DNA中扩增出线粒体DNA（mtDNA）的cyt.B基因、D环区基因和ITS基因。纯化的双链PCR产物用直接测序法进行测序。使用Kimra的2-parameter. ResultsD-环的方法估计遗传距离值，观察到最高的突变率之间的分析区域的皮尔彻德。由于D-loop的测序是为了确定当地的皮尔彻德种群结构，没有观察到明显的遗传差异之间收集的网站。因此，日本沿海沙丁鱼的种群结构可能是单一的。

This study was perform to elucidate the population structure of pilchard collected form coastal area of Japan.MethodsFish muscle (Sardinops melanostictus, Engraulis japonicus and Etrumeus teres) for total DNA extraction were collected from the coastal area of Japan. DNA was extracted from muscle of ethanol-preserved specimens using phenol-chloroform. The cyt.b gene and D-loop of the mitochondrial DNA (mtDNA) and ITS gene of gnome were amplified from total DNA using the polymerase chain reaction (PCR). The double strand PCR product purified were sequenced using direct sequencing method. Genetic distance values were estimated using the method of Kimra's 2-parameter.ResultsHighest mutation rate was observed in the D-loop among the analyzed regions of pilchards. Since D-loop was sequenced in order to identify the local population structure of pilchard, no obvious genetic differences were observed among the collected sites. Thus, single population structure is suggested in pilchard from coast of Japan.