Total analysis of adhesive protein genes from mussels.

研究了海洋、淡水贻贝和一种水生昆虫黏附蛋白的基因克隆。在淡水贻贝（Limnoperna fortunei）中，发现了一个fp-2样蛋白基因的完整序列。一种酶，酪氨酸酶，也被认为在贻贝足中表达。结果表明，淡水贻贝采用具有较高os Asp水平的fp样蛋白，说明静电相互作用是淡水环境中蛋白质聚集的重要力量之一，而DOPA的存在是保守的。这种水生昆虫有一个巨大的多肽，用于水泥和丝的形成。丝/水泥蛋白具有高水平的丝氨酸磷酸化残基，Ca2+与磷酸化蛋白之间的相互作用将驱动水泥/丝绸的形成。

Gene cloning of adhesive proteins from marine and freshwater mussels and one species of aquatic insect have been investigated. In the case of freshwater mussel, Limnoperna fortunei, a fp-2-like protein gene was found as an entire sequence. An enzyme, tyrosinase, was also suggested to be expressed in the mussel foot. The results indicated that freshwater mussels adopts the fp-like protein having higer level os Asp, which imply that electrostatic interactions is one of significant force for protein aggregation in freshwater environments, while presence of DOPA is conserved. The aquatic insect have a giant polypeptide for cement and silk formation. The silk/cement protein has high level of phosphorylated serine residues, and interaction between Ca2+ and the phosphorylated proteins will drive the cement/silk formation.