Development of the novel chemoselective capture method for sphingolipids

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To create new methodology of chemoselective catch toward sphingosine, large scale organic synthesis of target molecule was performed. Four standard samples having four different stereochemistries were prepared from D- and L-serine. The glutaraldehyde group was selected as functional group to react chemoselectively toward an 2-amino-1,3-diol function. The phenyl glutaraldehyde derivative was synthesized and connected with surface of the resin. The immobilization efficiency was confirmed by IR. The OPA derivatization method for detection of sphingosines reveals clearly its efficiency of the catching. To detect small amount of sphingosine in serum, downsizing of this new methodology was successfully performed utilizing the previously synthesize standard samples.