Development of low temperature liquid chromatography for comprehensive analysis of unstable compounds

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A chromatograph using liquid gas as a mobile phase was developed to achieve comprehensive analysis of unstable compounds in low temperature. The basic chromatographic behavior was studied mainly using a conventional ODS column with a mobile phase of liquid carbon dioxide at the temperature ranged from -35 to -5 ºC. The investigation revealed that both retention and separation efficiency behaviors varied specifically around -15ºC. That is, this result indicated that the possibility of phase transition of the stationary phase around this temperature. The analyses of quinone radical, which was generated by online electrochemical reaction, was also performed as a trial to analyze unstable species under low temperature and the variation in chromatogram was observed together with the electrochemical reaction.