Development ofporous multi-layer biomaterial and basic study on regeneration of cartilage-bone multi-layer tissue

本研究的主要目的是开发与天然软骨-骨层状组织具有生物力学相容性的多层支架，以应用于大规模关节损伤的再生医学。初步建立了再生人工骨组织的制备工艺。将生物可降解聚合物PCL和PLLA与生物陶瓷HA结合，成功地制备出了模拟软骨-骨层状组织力学性能的新型生物可吸收多层多孔材料。研究发现，分层支架的压缩性能与天然分层组织的压缩性能非常相似。再生的人工骨组织也通过将间充质干细胞与有机/无机复合支架培养一定时间而开发。

The primary aim of this study was to develop multi-layer scaffolds having biomechanical compatibility with natural cartilage-bone layered tissue in order to apply to regenerative medicine for large scale injuries of joints. Fundamental fabrication process for regenerated artificial bone tissue was also tried to be established. Novel bioabsorbable multi-layer porous materials imitating the mechanical properties of cartilage-bone layered tissue was successfully developed by using biodegradable polymers such as PCL and PLLA with bioceramic HA. It was found that the compressive properties of the layered scaffolds are very similar to those of natural layered tissues. Regenerated artificial bone tissues have also developed by culturing mesenchymal stem cells with organic/inorganic composite scaffold for a certain period.