Development of a Subcutaneous Site Islet Transplantation Method Using Cell Sheet.

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We aimed to establish a method to transplant islets of islets to subcutaneous sites with pre-constructed vascular beds by using cell sheets. We found fat-derived stem cells are more appropriate for vascular bed construction prior to subcutaneoustransplantation than basic fibroblast growth factors (bFGF) entailing bloody effusions. In addition, we succeeded in preparing islet cell sheets consisting of islet cell sheets and fibroblasts and in an in vitro sugar tolerance test we confirmed insulin secretion accompanying increase in glucose concentration . However, we could not produce islet cell sheets containing an enough amount of islets to curediabetic animals.