Investigation into the mechanism of mechano-transduction via neurotransmitter receptors and ion channels

通过神经递质受体和离子通道研究机械传导机制

• 批准号: 23791626
• 负责人: NOTOMI Takuya
• 金额: $ 2.75万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23791626/
• 关键词: 膜電位; 骨代謝; 破骨細胞; イオンチャネル

Bone diseases are serious problem in an aged society and their treatment is necessary for maintaining QOL. To prevent bone diseases, we have done the study of effects of ion channels and membrane potential in bone. To investigate the signaling mechanism via ion channel and receptor, we examined the function of bone cells by light-sensitive ion channel and pump. The light-gated ion channels and pump were transfected into bone cells and then the role of membrane potential on those cells were investigated. Further, based on their results, we identified bone remodeling-related novel ion channels. In summary, our results indicate that membrane potential regulate bone cell function and bone remodeling-related novel ion channels are important for osteoclastic functions.

骨骼疾病是老龄化社会的严重问题，其治疗是维持生活质量的必要条件。为了预防骨疾病，我们研究了骨中离子通道和膜电位的作用。为探讨光敏感离子通道和光敏感离子泵在骨细胞中的作用机制，本研究采用光敏感离子通道和光敏感离子泵检测骨细胞的功能。将光门控离子通道和光门控离子泵转染骨细胞，观察膜电位对骨细胞的影响。此外，基于他们的结果，我们确定了骨重建相关的新离子通道。总之，我们的研究结果表明，膜电位调节骨细胞的功能和骨重建相关的新的离子通道是重要的骨功能。

项目成果

一次繊毛タンパクBbs3は骨代謝に関与する

初级纤毛蛋白Bbs3参与骨代谢

• 发表时间: 2012
• 作者: 川崎真希理;中元哲也;納富拓也;早田匡芳;江面陽一;野田政樹
• 通讯作者: 野田政樹

PTH Enhances Mechanical Stress-induced Osteoblast Proliferation in Calvarial Derived Osteoblasts via Up-regulation of CyclinD1 Expression

PTH 通过上调 CyclinD1 表达增强颅骨来源的成骨细胞中机械应力诱导的成骨细胞增殖

• 发表时间: 2012
• 作者: Shirakawa Jumpei;Yoichi Ezura;TakuyaNotomi;Tadayoshi Hayata;Tetsuya Nakamoto;Shuichi Moriya;Ken Omura;Masaki Noda
• 通讯作者: Masaki Noda

Opto-functional control of intracellular RANKL transport/secretion in osteoblasts to enhance coupling with osteoclasts based on photo-energy conversion of molecularly-engineered channel-rhodopsin

基于分子工程通道视紫红质的光能转换，对成骨细胞中的细胞内 RANKL 运输/分泌进行光功能控制，以增强与破骨细胞的偶联

• 发表时间: 2011
• 作者: Takuya Notomi;Miyuki Kuno;Yoichi Ezura;Masaki Noda
• 通讯作者: Masaki Noda

Parathyroid Hormone Stimulates Tob1Expression in Osteoblastic Cells in vitro and in vivo

甲状旁腺激素在体外和体内刺激成骨细胞中 Tob1 的表达

• 发表时间: 2012
• 作者: Shuichi Moriya;Tadayoshi Hayata;Jumpei Shirakawa;Tetsuya Nakamoto;Takuya Notomi;Yoichi Ezura;Kazuo Kaneko;Masaki Noda
• 通讯作者: Masaki Noda

Cnot3 (Ccr4-not complex subunit3), a Regulator of mRNA Stability, Regulates Bone Mass and Gene Expression Related to Osteoclast Formation

Cnot3（Ccr4-非复合亚基 3）是 mRNA 稳定性的调节因子，调节骨量和与破骨细胞形成相关的基因表达

• 发表时间: 2012
• 作者: Chiho Watanabe;Masahiro Morita;Yoichi Ezura;Tetsuya Nakamoto;Tadayoshi Hayata;Takuya Notomi;Keiji Moriyama;Tadashi Yamamoto;Masaki Noda
• 通讯作者: Masaki Noda