Influence of the anesthetic on nerve circuit construction of the period of growth by the visualization of the GABAergic neuron

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All procedures were performed on adult (12-16 weeks of age) male wild-type(WT) and GAD65 knockout mice (GAD65-/-).Three pain-related behavior tests(Hotplate test; Tail-immersion test; Formaline test) were performed to assess response to nociceptive stimulation. We measured latencies to licking or jumping from the hotplate set at53centigrade A significant alteration of the response was found in male GAD65 (-/-) (p<0.01)compared to their wild-type controls.indicative of increased nociceptive perception. However, there was no significant difference in the latency of the tail-immersion test, which was known to spinally-mediated nociceptive thresholds. In formalin test,there were no significant difference in peak response time of phaseI and phaseII,but GAD65(-/-)mice indicate the prolongation of hepersensitivity in phaseII