Influence of the anesthetic on nerve circuit construction of the period of growth by the visualization of the GABAergic neuron

所有实验均在成年（12-16周龄）雄性野生型（WT）和GAD65基因敲除小鼠（GAD65-/-）上进行。采用三种疼痛相关行为测试（热板测试、尾浸测试、福尔马林测试）评估痛觉刺激的反应。我们测量了舔舐或从53℃的热板上跳下来的潜伏期。与野生型对照相比，GAD65雄性小鼠的反应发生了显著变化（-/-）（p<0.01）。表明伤害性知觉增强。然而，尾巴浸入测试的潜伏期没有显著差异，这是已知的脊髓介导的伤害感受阈值。在福尔马林试验中，第i期和第ii期的峰值反应时间无显著差异，但GAD65（-/-）小鼠显示第ii期肝敏感性延长

All procedures were performed on adult (12-16 weeks of age) male wild-type(WT) and GAD65 knockout mice (GAD65-/-).Three pain-related behavior tests(Hotplate test; Tail-immersion test; Formaline test) were performed to assess response to nociceptive stimulation. We measured latencies to licking or jumping from the hotplate set at53centigrade A significant alteration of the response was found in male GAD65 (-/-) (p<0.01)compared to their wild-type controls.indicative of increased nociceptive perception. However, there was no significant difference in the latency of the tail-immersion test, which was known to spinally-mediated nociceptive thresholds. In formalin test,there were no significant difference in peak response time of phaseI and phaseII,but GAD65(-/-)mice indicate the prolongation of hepersensitivity in phaseII