A prediction and a therapeutic strategy for lung hypoplasia in a rat CDH model

大鼠CDH模型肺发育不全的预测和治疗策略

• 批准号: 23791826
• 负责人: TSUDA HIROYUKI
• 金额: $ 2.66万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23791826/
• 关键词: 横隔膜ヘルニア; 肺低形成; ラメラ体; 羊水; 予防; 柴苓湯

We initially enrolled 19 cases diagnosed with CDH prenatally. LBC was significantly higher in the surviving group than in deceased group (p < 0.01).In the rat experiment, we collected amniotic fluid and lung tissue from newborns at E21 and confirmed the presence of lamellar bodies in both the amniotic fluid. We also confirmed a similar lamellar body size in amniotic fluid and type II alveolar cells comparable to the lamellar body findings in human amniotic fluid. Next, after exposure of pregnant rats to a 100 mg dose of nitrofen, 48.8% (61/125) of newborns had diaphragmatic hernias. LBC was significantly higher in control than in CDH (p < 0.01) rats. Furthermore, lung weight was also significantly higher in control than in CDH (p < 0.01) newborn rats. We examined the expression of ABCA3 in lung tissues from newborn rats, delivered at E21, by Western blotting. ABCA3 expression did not differ significantly between controls and rats with CDH.

我们最初纳入了19例产前诊断为先天性髋关节脱位的病例。存活组LBC显著高于死亡组（P < 0.01）。在大鼠实验中，我们收集了E21新生儿的羊水和肺组织，证实羊水和肺组织中均存在板层小体。我们还证实了类似的板层体大小在羊水和II型肺泡细胞的板层体在人类羊水中的发现。接下来，在将怀孕大鼠暴露于100 mg剂量的除草醚后，48.8%（61/125）的新生儿患有腹股沟疝。对照组LBC显著高于CDH组（p < 0.01）。此外，对照组新生大鼠的肺重量也显著高于CDH组（p < 0.01）。我们检测了ABCA 3的表达在肺组织中的新生大鼠，交付E21，通过蛋白质印迹。ABCA 3表达在对照组和CDH大鼠之间没有显著差异。

项目成果

Amniotic lamellar body counts and congenital diaphragmatic hernia in humans and in a rat model

人类和大鼠模型中的羊膜板层计数和先天性膈疝

• 发表时间: 2013
• 期刊: Pediatr Res
• 影响因子: 3.6
• 作者: Watanabe Y;Tsuda H;Kotani T;Sumigama S;Mano Y;Hayakawa M;Sato Y;Kikkawa F
• 通讯作者: Kikkawa F

NEONATAL CARE Vol.26 先天性横隔膜ヘルニア

新生儿护理 Vol.26 先天性膈疝

• 发表时间: 2013
• 作者: 津田弘之;炭竈誠二;伊藤美春;早川昌弘
• 通讯作者: 早川昌弘

Amniotic lamellar body counts and congenital diaphragmatic hernia in humans and in a rat model.

人类和大鼠模型中的羊膜板层计数和先天性膈疝。

• 发表时间: 2013
• 期刊: Pediatric Research
• 影响因子: 3.6
• 作者: Watanabe Y;Tsuda H;Kotani T;Sumigama S;Mano Y;Hayakawa M;Sato Y;Kikkawa F.
• 通讯作者: Kikkawa F.

先天性横隔膜ヘルニアの予後羊水中ラメラ体を使用して

利用羊水板层体预测先天性膈疝

• 发表时间: 2013
• 作者: 渡部百合子;津田弘之;澤田雅子;諸井博明;今井健史;松川哲;中野知子;服部友香;真野由紀雄;炭竈誠二;小谷友美;吉川史隆
• 通讯作者: 吉川史隆

Amniotic lamellar body count and congenital diaphragmatic hernia in humans and in a rat model.

人类和大鼠模型中的羊膜板层计数和先天性膈疝。

• DOI: 10.1038/pr.2012.187
• 发表时间: 2013
• 期刊: Pediatr Res
• 影响因子: 3.6
• 作者: Watanabe Y;Tsuda H;Kotani T;Sumigama S;Mano Y;Hayakawa M;et al.
• 通讯作者: et al.