Analysis of retina degeneration protection mechanism by the Channelrodopsin gene introduction.

视紫红质通道蛋白基因导入对视网膜变性的保护机制分析

• 批准号: 23791961
• 负责人: ISAGO Hitomi
• 金额: $ 2.75万
• 依托单位: Iwate University (2012)Tohoku University (2011)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23791961/
• 关键词: 眼生理学; 視覚再生; 視覚神経学; 神経科学

We examined protection effects of ChR2 expression on the retinal ganglion cells of hereditary photoreceptor cell degeneration rats(RCS rats). The RCS rat showed recovery of visual evoked potentials after the gene transfer, that indicated the function of the ganglion cell was held. Moreover, results of the immunohistological evaluation indicated ChR2 was also effective on secondary degeneration of ganglion cells by the ChR2 expression.Next, mRNAs from the retinas were extracted and performed by a microarray analysis. As a result, we detected 437 increased-genes and 755 decreased-genes in the ChR2 transduced group. The detected genes were relevant to ion channels and signal transductions.It was thought that these changes were caused by ChR2 gene functioned as a photoactivated ion channel within the retina after ChR2 gene transfer.

我们研究了ChR2表达对遗传性感光细胞变性大鼠（RCS）视网膜神经节细胞的保护作用。基因转染后RCS大鼠视觉诱发电位恢复，表明神经节细胞功能保持。此外，免疫组织学评价结果表明，ChR2通过表达对神经节细胞继发性变性也有效。接下来，从视网膜中提取mrna并进行微阵列分析。结果，我们在ChR2转导组中检测到437个增加基因和755个减少基因。检测到的基因与离子通道和信号转导有关。这些变化被认为是由于ChR2基因转移后在视网膜内作为光激活离子通道而引起的。

项目成果

Intravitreal injection or topical eye-drop application of a μ-calpain C2L domain peptide protects against photoreceptor cell death in Royal College of Surgeons' rats, a model of retinitis pigmentosa

• DOI: 10.1016/j.bbadis.2012.07.018
• 发表时间: 2012-11-01
• 期刊: BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
• 影响因子: 6.2
• 作者: Ozaki, Taku;Nakazawa, Mitsuru;Ishiguro, Sei-ichi
• 通讯作者: Ishiguro, Sei-ichi

視覚再生研究の新たな展開

视觉再现研究新进展

• 发表时间: 2011
• 作者: 富田浩史;菅野江里子;砂金ひとみ
• 通讯作者: 砂金ひとみ

Differentiation of neuronal cells from NIH/3T3 fibroblasts under defined conditions

• DOI: 10.1111/j.1440-169x.2010.01235.x
• 发表时间: 2011-04
• 期刊: Development
• 影响因子: 4.6
• 作者: Zhuo Wang;E. Sugano;H. Isago;T. Hiroi;M. Tamai;H. Tomita

Ishiguro SI, Intravitreal injection or topical eye-drop application of a μ-calpain C2L domain peptide protects against photoreceptor cell death in Royal College of Surgeons rats a model of retinal pgmentosa

Ishiguro SI，玻璃体内注射或局部滴眼液使用 μ-钙蛋白酶 C2L 结构域肽可防止皇家外科学院大鼠视网膜色素变性模型中的感光细胞死亡

• 发表时间: 2012
• 期刊: Biochemica et Biophysica Acta
• 作者: Ozaki T;Nakazawa M;Yamashita T;Sorimachi H;Hata S;Tomita H;Isago H;Baba A
• 通讯作者: Baba A

Improvement Of Wavelength Sensitivities By The Modification Of Volvox Channelrhodopsin-1 Gene

通过修饰Volvox通道视紫红质-1基因提高波长敏感性

• 发表时间: 2011
• 作者: Tomita H;Sugano E;Isago H;Murayama N;Wang Z;Tamai M
• 通讯作者: Tamai M