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"What is the keloid diathesis? "-Role of Fibrocytes in Keloids -

“什么是疤痕疙瘩素质？”-纤维细胞在疤痕疙瘩中的作用-

基本信息

批准号：
23792051
负责人：
NAGAO Munetomo
金额：
$ 2.66万
依托单位：
Iwate Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Young Scientists (B)
财政年份：
2011
资助国家：
日本
起止时间：
2011 至 2012
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23792051/
关键词：
創傷治癒学ケロイド創傷治癒 fibrocyte 線維化

项目摘要

Keloids are often difficult to treat . The mechanisms underlying keloid formation are only partially understood. Because multiple and recurrent lesions frequently occur in patients with keloids, we hypothesize that systemic abnormalities are involved in the pathogensis. We analyzed fibroblast progenitor cells, called “circulating fibrocytes(CFs)”, in the peripheral blood of keloid patients. The altered characteristics of CFs from patients with keloids may have an important role in the pathogenesis of keloids. The aim of this study, Blood samples were obtained from the pat ients with keloids, and the healthy volunteers. To isolate CFs from peripheral blood, peripheral blood mononuclear cells (PBMCs) were separated from whole blood (10ml ~20ml) by Ficoll Hypaque density -gradient centrifugation, and cultured in DMEM. On the other hand, the tissue from keloids patients were cultured with previous methods and isolated fibroblasts. Before that time , we made experiment in vivo for the relation between the wound healing and fibrocytes using NOD scid mice. We injured for the back of NOD scid mice, making the φ5mm skin defects, and then injected the human CFs from the tail vein. Imunohis tochemistry was performed from the back scar tissue of the mice. CFs stained human HLA -a,b,c antibody from the scar tissue after 7days. The mRNA expression of humanGAPDH, humanTGF -β1 were increased after 14days, compared with normal skin.CFs were migrate in the wound site with expression of the various cytokines. And influence on wound healing. We will able to control of the abnormal CFs, its might be useful for the treatment for scar formation.

疤痕疙瘩通常很难治疗。瘢痕疙瘩形成的机制目前尚不完全清楚。由于瘢痕疙瘩患者经常出现多发性和复发性病变，我们推测全身异常参与了发病机制。我们分析了瘢痕疙瘩患者外周血中的成纤维细胞祖细胞，称为“循环纤维细胞（CF）”。疤痕疙瘩患者 CF 特征的改变可能在疤痕疙瘩的发病机制中发挥重要作用。本研究的目的是从疤痕疙瘩患者和健康志愿者身上采集血液样本。为了从外周血中分离CF，通过Ficoll Hypaque密度梯度离心从全血（10ml~20ml）中分离外周血单核细胞（PBMC），并在DMEM中培养。另一方面，用以前的方法培养来自疤痕疙瘩患者的组织并分离出成纤维细胞。在此之前，我们利用NOD scid小鼠进行了体内伤口愈合与纤维细胞关系的实验。我们对NOD scid小鼠背部进行损伤，制作φ5mm的皮肤缺损，然后从尾静脉注射人CFs。对小鼠背部疤痕组织进行免疫组织化学分析。 7 天后，CF 对疤痕组织中的人类 HLA -a、b、c 抗体进行染色。 14天后，与正常皮肤相比，人GAPDH、人TGF-β1的mRNA表达增加。CFs在伤口部位迁移，并表达各种细胞因子。并影响伤口愈合。我们将能够控制异常的CF，它可能有助于治疗疤痕形成。