"What is the keloid diathesis? "-Role of Fibrocytes in Keloids -

瘢痕疙瘩通常很难治疗。瘢痕疙瘩形成的机制只有部分了解。由于多发性和复发性病变经常发生在瘢痕疙瘩患者，我们假设，全身异常参与发病机制。我们分析了瘢痕疙瘩患者外周血中的成纤维祖细胞，称为“循环纤维细胞（CF）”。瘢痕疙瘩患者CFs特征的改变可能在瘢痕疙瘩的发病机制中起重要作用。本研究的目的是从瘢痕疙瘩患者和健康志愿者中获得血液样品。采用Ficoll Hypaque密度梯度离心法从10 ml ~ 20 ml全血中分离外周血单个核细胞（PBMC），并在DMEM中培养。另一方面，瘢痕疙瘩患者的组织用先前的方法培养并分离成纤维细胞。在此之前，我们用NOD scid小鼠进行了创伤愈合与纤维细胞关系的体内实验。对NOD scid小鼠背部造成损伤，造成φ 5 mm皮肤缺损，从尾静脉注射人CFs。从小鼠背部瘢痕组织进行免疫组化。7天后，CFs染色人HLA-a、B、c抗体。与正常皮肤相比，伤后14 d，人GAPDH、人TGF-β1 mRNA表达增加，CFs在创面迁移，表达多种细胞因子。以及对伤口愈合的影响。我们将能够控制异常的CFs，这可能有助于治疗瘢痕形成。

Keloids are often difficult to treat . The mechanisms underlying keloid formation are only partially understood. Because multiple and recurrent lesions frequently occur in patients with keloids, we hypothesize that systemic abnormalities are involved in the pathogensis. We analyzed fibroblast progenitor cells, called “circulating fibrocytes(CFs)”, in the peripheral blood of keloid patients. The altered characteristics of CFs from patients with keloids may have an important role in the pathogenesis of keloids. The aim of this study, Blood samples were obtained from the pat ients with keloids, and the healthy volunteers. To isolate CFs from peripheral blood, peripheral blood mononuclear cells (PBMCs) were separated from whole blood (10ml ~20ml) by Ficoll Hypaque density -gradient centrifugation, and cultured in DMEM. On the other hand, the tissue from keloids patients were cultured with previous methods and isolated fibroblasts. Before that time , we made experiment in vivo for the relation between the wound healing and fibrocytes using NOD scid mice. We injured for the back of NOD scid mice, making the φ5mm skin defects, and then injected the human CFs from the tail vein. Imunohis tochemistry was performed from the back scar tissue of the mice. CFs stained human HLA -a,b,c antibody from the scar tissue after 7days. The mRNA expression of humanGAPDH, humanTGF -β1 were increased after 14days, compared with normal skin.CFs were migrate in the wound site with expression of the various cytokines. And influence on wound healing. We will able to control of the abnormal CFs, its might be useful for the treatment for scar formation.