A perception reproduction mechanism in a flap and a skin graft , and a new possibility of the tacrolimus external use

皮瓣和植皮的感知再现机制及他克莫司外用的新可能性

• 批准号: 23792060
• 负责人: NATORI Yuhei
• 金额: $ 2.75万
• 依托单位: Juntendo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23792060/
• 关键词: 皮弁; 植皮術; 神経再生; トランスジェニックマウス; live imaging; FK506; 短期的神経刺激

To evaluating sensory innervation into either skin flap or skin graft, we used transgenic mice which all axons express YFP fluorescent and developed live imaing system for murine skin flap model. It was useful tool for evaluating sens ory innervation and the intensity is elevated only at the flap which sensory nerve was preserved. We also evaluated the effect of applying tacrolimus ointment on the flap, and the tacrolimus applied group showed higher intensity than the other groups. The application of the tacrolimus may promote sensory nerve regeneration into the flap.

为了评价皮瓣或皮片的感觉神经支配，我们使用所有轴突都表达YFP荧光的转基因小鼠，并开发了小鼠皮瓣模型的活体成像系统。该方法是评价感觉神经分布的有效工具，仅在保留感觉神经的皮瓣上感觉神经强度增加。我们还评估了在皮瓣上应用他克莫司软膏的效果，他克莫司应用组显示出比其他组更高的强度。应用他克莫司可促进感觉神经再生入皮瓣。