Establishment of m olecular and structural basis of hydrogen sulfide-producing mechanism in Fusobacteriu m

梭杆菌产生硫化氢机制的分子和结构基础的建立

• 批准号: 23792130
• 负责人: KEZUKA Yuichiro
• 金额: $ 2.75万
• 依托单位: Iwate Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23792130/
• 关键词: 口腔生化学; 硫化水素産生; 歯周病; 酵素; 立体構造; 硫化水素; Fusobacterium

Hydrogen sulfide produced by oral bacteria is a predominant causative agent of oral malodor and is also known to be a pathogenic factor of periodontal disease. In this research, we determined the structures of two hydrogen sulfide-producing enzymes in Fusobacterium nucleatum, a periodontal pathogen that can produce a significant amount of hydrogen sulfide in human oral cavity. Furthermore, we analyzed enzymatic properties of the wild types and site -directed mutants. Based on the results , the reaction mechanisms of the enzymes were elucidated, and molecular and structural basis of hydrogen sulfide-producing mechanism in this oral bacterium could be established.

口服细菌产生的硫化氢是口服癌的主要病因，也已知是牙周疾病的致病因素。在这项研究中，我们确定了核细菌中两种产生硫化氢酶的结构，核细菌是一种牙周病原体，可以在人口腔中产生大量的硫化氢。此外，我们分析了野生类型和位置突变体的酶促性质。根据结果​​，可以阐明酶的反应机制，并可以建立该口腔细菌中产生硫化氢机制的分子和结构基础。

项目成果

Purification, crystallization and preliminary X-ray analysis of two hydrogen sulfide-producing enzymes from Fusobacterium nucleatum

具核梭杆菌两种产硫化氢酶的纯化、结晶和初步 X 射线分析

• DOI: 10.1107/s1744309112042546
• 发表时间: 2012
• 期刊: Acta Crystallogr Sect F Struct Biol Cryst Commun
• 作者: Kezuka Y;Abe N;Yoshida Y;Nonaka
• 通讯作者: Nonaka

Identification and enzymatic analysis of a novel protein associated with production of hydrogen sulfide and L-serine from L-cysteine in

与从 L-半胱氨酸产生硫化氢和 L-丝氨酸相关的新型蛋白质的鉴定和酶分析

• 发表时间: 2011
• 期刊: Fusobacterium nucleatum subsp. nucleatum ATCC 25586
• 作者: Yoshida;Y.;Suwabe;K.;Nagano;K.;Kezuka;Y.;Kato;H. and Yoshimura;F
• 通讯作者: F

歯周病原細菌由来硫化水素産生酵素-反応中間体の結晶構造

牙周病原菌产生硫化氢的酶-反应中间体的晶体结构

• 发表时间: 2013
• 作者: 毛塚雄一郎;吉田康夫;野中孝昌
• 通讯作者: 野中孝昌

蛋白質のX線構造解析と応用

蛋白质X射线结构分析及应用

• 发表时间: 2011
• 作者: 津村麻記;Sobhan Ubaidus;佐藤正樹;西山明宏;田崎雅和;澁川義幸;Uehara et al;野中孝昌
• 通讯作者: 野中孝昌

Structure analysis of hydrogen sulfide-produicing enzyme complexed with its reaction intermediates from a periodontal pathogen

牙周病原菌产生硫化氢的酶及其反应中间体的结构分析

• 发表时间: 2012
• 作者: Kezuka;Y.;Yoshida;Y. and Nonaka;T
• 通讯作者: T