Basic research of stem cell therapy for radiation damage of oral and maxillofacial region.

干细胞治疗口腔颌面部放射损伤的基础研究。

• 批准号: 23792319
• 负责人: ABE Shigehiro
• 金额: $ 2.66万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23792319/
• 关键词: 幹細胞; 分化; Neurosphere; Tissue engineering; 歯髄幹細胞; 口腔粘膜固有層幹細胞; 神経堤幹細胞; 骨再生; 再生医学; 放射線生物学; Ｔｉｓｓｕｅ Ｅｎｇｉｎｅｅｒｉｎｇ

Previously, we demonstrated for the first time that stem/progenitor cells derived from apicalpapilla-derived cells (APDCs) exhibit a radioresistant phenotype; however, the hard tissue forming ability in vivo, but not bulk APDCs, was significantly reduced after irradiation. ( S. Abe et al. Stem Cell Res Therap, 2011.)The purpose of this study was to isolate and characterize the properties of stem/progenitor cells derived from human APDCs and human oral mucosal mesenchymal tissue-derived cells (OMMCs).APDCs and OMMCs were isolated from freshly extracted human third molars with immature apices and human oral mucosal tissues (lip, palate, gingiva and alveolar ridge). APDCs and OMMCs formed spheres under neurosphere culture condition. The sphere-forming cells derived from APDCs and OMMCs had expressed neural crest-associated markers. These sphere-forming cells could differentiate into multiple mesenchymal lineages (osteoblasts, adipocytes and chondrocytes ) and neural lineage (neurons) in vitro, and generated ectopic bone tissues in vivo. The results of this study suggest that APDCs and OMMCs contain cells with characteristics of NCSCs reported previously in mice. Humans developing tooth with immature apex and human oral mucosal mesenchymal tissue-derived cells are the effective source of cells for neural crest lineage tissue regeneration.

在此之前，我们第一次证明了来自apicalpapilla-derived细胞（APDC）的干/祖细胞表现出抗辐射表型;然而，在体内的硬组织形成能力，但不是散装APDC，照射后显着降低。（S. Abe等，Stem Cell Res Therap，2011.）本研究从人新鲜拔除的根尖未成熟的第三磨牙和人口腔黏膜组织（唇、腭、牙龈和牙槽嵴）中分离人牙源性树突状细胞（APDCs）和口腔黏膜间充质组织来源细胞（OMMCs），并对其干/祖细胞进行分离和鉴定。在神经球培养条件下，APDCs和OMMCs形成球形。来源于APDC和OMMC的成球细胞表达神经嵴相关标志物。这些成球细胞在体外可分化为多种间充质细胞（成骨细胞、脂肪细胞和软骨细胞）和神经细胞（神经元），并在体内产生异位骨组织。这项研究的结果表明，APDC和OMMC包含的细胞与NCSC的特征以前在小鼠中报道。人类发育中的根尖未成熟牙齿和人口腔黏膜间充质组织来源的细胞是神经嵴谱系组织再生的有效细胞来源。

项目成果

上顎劣成長を伴った片側性完全口唇口蓋裂患者に対する上下顎移動術および骨延長術の顎顔面形態と後戻りに関する比較検討

单侧完全性唇腭裂伴上颌骨生长不足患者颌面形态及上颌运动回归及骨延长的对比研究

• 发表时间: 2012
• 期刊: 日本口腔外科学会雑誌
• 作者: 阿部成宏;吉増秀實;佐藤豊;藤村倫子;三島木節;村嶋真由子;香月佑子;上丸英;新井直也;天笠光雄
• 通讯作者: 天笠光雄

ヒト根未完成歯・根尖部歯髄由来細胞の放射線生物学的検討

人类不完整牙根牙髓细胞的放射生物学研究

• 发表时间: 2011
• 作者: 阿部成宏;濱田啓一;山口聰;天笠光雄;山城正司
• 通讯作者: 山城正司

Long term follow up results of the patients with cleft lip and palate

唇腭裂患者长期随访结果

• 发表时间: 2012
• 作者: M.Murashima;T. Mishimagi;S. Abe;S. Okada;Y. Katsuki;S. Iijima;Y. Sato;H. Yoshimasu;M. Mibu;K. Harada
• 通讯作者: K. Harada

Long term follow up results of the patients with cleft lip and palate.

唇腭裂患者的长期随访结果。

• 发表时间: 2012
• 作者: M.Murashima;T. Mishimagi;S. Abe;S. Okada;Y. Katsuki;S. Iijima;Y. Sato;H. Yoshimasu;M. Mibu;K. Harada
• 通讯作者: K. Harada