The analysis of the carcinogenesis and aquired immunity in the oral mucosa lesion

口腔黏膜病变的癌变及获得性免疫分析

• 批准号: 23792391
• 负责人: KUMAGAI Kenichi
• 金额: $ 2.66万
• 依托单位: Tsurumi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23792391/
• 关键词: 口腔粘膜; 白板症; 癌化; 癌幹細胞; 口腔粘膜疾患; ErbB family gene; 口腔白板症; 口腔扁平苔癬

The expression of four epidermal growth factor receptor (EGFR) family genes and their ligands were measured in LP tissues from 14 patients and compared with levels in 10 patients with oral lichen planus (OLP) and normal oral mucosa (NOM) from 14 healthy donors by real-time polymerase chain reaction (PCR) and immunohistochemistry. Synchronous mRNA coexpression of ErbB1, ErbB2, ErbB3 and ErbB4 was detected in LP lesions. Out of the receptors, only ErbB4 mRNA and protein was more highly expressed in LP compared with NOM tissues. These were strongly expressed by epithelial keratinocytes in LP lesions, as shown by immunohistochemistry. Regarding the ligands, the mRNA of Neuregulin2 and 4 were more highly expressed in OLP compared with NOM tissues. Therefore, enhanced ErbB4 on the keratinocytes and synchronous modulation of EGFR family genes may contribute to the pathogenesis and carcinogenesis of LP.

采用实时定量聚合酶链式反应(PCR)和免疫组织化学方法检测了14例扁平苔藓(LP)组织中4种表皮生长因子受体(EGFR)家族基因及其配体的表达，并与10例口腔扁平苔藓(OLP)和14例正常口腔黏膜(NOM)进行了比较。皮损中ErbB1、ErbB2、ErbB3和ErbB4的mRNA同步共表达。在受体中，只有ErbB4mRNA和蛋白在LP组织中的表达高于正常组织。免疫组织化学结果显示，扁平苔藓皮损上皮角质形成细胞呈强阳性表达。在配体方面，口腔扁平苔藓组织中NeuRegin2和NeuRegin4mRNA的表达水平明显高于正常口腔黏膜组织。因此，角质形成细胞表面ErbB4的表达增强和EGFR家族基因的同步调控可能参与了LP的发生发展过程。

项目成果

Clinicopathological significance and prognostic value of CD133 expression in oral squamous cell carcinoma 64

CD133表达在口腔鳞状细胞癌中的临床病理意义及预后价值 64

• 发表时间: 2014
• 作者: Kenichi Kumagai;Tsuyoshi Amemiya;Koji Kawaguchi;Ryuji Suzuki;Keisuke Fujii;Hiroaki Shigematsu;Hiroyuki Yamada;Yoshiki Hamada
• 通讯作者: Yoshiki Hamada

口腔白板症および口腔扁平苔癬の病態形成に関わるEGFR familyの発現解析

口腔白斑及口腔扁平苔藓发病机制中EGFR家族的表达分析

• 发表时间: 2011
• 作者: 熊谷賢一;藤井恵介;北浦一孝;江口貴紀;重松宏昭;岸悠太;山田浩之;川口浩司;鈴木隆二;濱田良樹;小林浩,熊谷賢一,江口貴紀,重松宏昭,和気昌宏,濱田良樹
• 通讯作者: 小林浩,熊谷賢一,江口貴紀,重松宏昭,和気昌宏,濱田良樹

Clinicopathological significance and prognostic value of CD133 expression in oral squamous cell carcinoma Journal of Oral and Maxillofacial Surgery

口腔鳞状细胞癌中CD133表达的临床病理意义及预后价值 口腔颌面外科杂志

• 发表时间: 2014
• 期刊: Medicine and Pathology
• 作者: Keisuke Fujii;Kenichi Kumagai;Ryuji Suzuki;Yoshiki Hamada
• 通讯作者: Yoshiki Hamada

口腔癌の頚部リンパ節転移における腫瘍免疫応答の解明

阐明口腔癌颈部淋巴结转移中的肿瘤免疫反应

• 发表时间: 2012
• 作者: 熊谷賢一;藤井恵介;北浦一孝;江口貴紀;重松宏昭;岸悠太;山田浩之;川口浩司;鈴木隆二;濱田良樹
• 通讯作者: 濱田良樹

Synchronous modulation of epidermal growth factor receptor family genes in oral premalignant lesions

口腔癌前病变中表皮生长因子受体家族基因的同步调节

• 发表时间: 2011
• 作者: Kenichi Kumagai;Hiroshi Kobayashi;Akito Gotoh;Takanori Eguchi;Hiroyuki Yamada;Ryuji Suzuki;Yoshiki Hamada
• 通讯作者: Yoshiki Hamada