The analysis of the carcinogenesis and aquired immunity in the oral mucosa lesion

采用实时聚合酶链反应（PCR）和免疫组织化学方法检测14例口腔扁平苔藓（OLP）和14例正常口腔黏膜（NOM）患者的4个表皮生长因子受体（EGFR）家族基因及其配体的表达，并与10例口腔扁平苔藓（OLP）和正常口腔黏膜（NOM）的表达进行比较。在LP病变中检测到ErbB1、ErbB2、ErbB3和ErbB4 mRNA同步共表达。在这些受体中，只有ErbB4 mRNA和蛋白在LP中的表达高于NOM组织。免疫组织化学显示，这些蛋白在LP病变的上皮角质形成细胞中强烈表达。在配体方面，与NOM组织相比，OLP组织中Neuregulin2和4 mRNA的表达量更高。因此，角化细胞上ErbB4的增强和EGFR家族基因的同步调节可能参与了LP的发病和癌变。

The expression of four epidermal growth factor receptor (EGFR) family genes and their ligands were measured in LP tissues from 14 patients and compared with levels in 10 patients with oral lichen planus (OLP) and normal oral mucosa (NOM) from 14 healthy donors by real-time polymerase chain reaction (PCR) and immunohistochemistry. Synchronous mRNA coexpression of ErbB1, ErbB2, ErbB3 and ErbB4 was detected in LP lesions. Out of the receptors, only ErbB4 mRNA and protein was more highly expressed in LP compared with NOM tissues. These were strongly expressed by epithelial keratinocytes in LP lesions, as shown by immunohistochemistry. Regarding the ligands, the mRNA of Neuregulin2 and 4 were more highly expressed in OLP compared with NOM tissues. Therefore, enhanced ErbB4 on the keratinocytes and synchronous modulation of EGFR family genes may contribute to the pathogenesis and carcinogenesis of LP.