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ブナ科樹木萎凋病原菌に対する宿主の抵抗性に関わる因子の解明

阐明宿主对壳斗科树枯病病原体的抗性相关因素

基本信息

批准号：
11J07385
负责人：
鳥居正人
金额：
$ 1.22万
依托单位：
Mie University
依托单位国家：
日本
项目类别：
Grant-in-Aid for JSPS Fellows
财政年份：
2011
资助国家：
日本
起止时间：
2011 至 2013
项目状态：
已结题

项目摘要

変色域の形成程度により, Raffaelea quereivoraの宿主樹木に対する病原力は菌株間で異なると指摘されているが, 枯死の程度や枯死に関わる非通水域の形成程度による評価は行われていない. また, 非通水域の拡大に関与する材内における本菌の菌糸伸長と病原力との関連も明らかではない. そこで本研究では, R. quercivora菌株間の病原力の差異と, その差異と材内における菌糸伸長との関連を明らかにすることを目的とした. 変色域の形成に関わるタンナーゼとラッカーゼ活性を, タンニン酸かABTS添加培地における変色程度で評価した. 日本全国から採取した32菌株の中から, 両酵素活性が異なる4菌株と9菌株を選抜し, それぞれミズナラ苗木に多点, 成木の枝に1点接種を行い, 枯死率と横断面に占める非通水域の割合を算出した. さらに, 1点接種においては材内における菌糸伸長量を計測した. その結果, 菌株間で両酵素活性と枯死率, 非通水域の割合, 菌糸伸長量は有意に異なった. さらに, 両酵素活性と枯死率, 非通水域の割合との間に関連はみられなかったが, 菌株間の菌糸伸長量の違いは, 枯死率や非通水域の割合の違いと一致する傾向にあった. 以上より, 本菌の病原力は菌株間で異なるが, 両酵素活性の関与は極めて低いと示唆された. さらに, 菌株間の病原力の差異には, 材内における菌糸伸展の程度が関与すると示唆された.現在までに行った研究の一部を, 博士論文として取りまとめた.

The degree of formation of the color gamut, the degree of formation of the Raffaelea quereivora host, the pathogenicity of the strain, the degree of death, the degree of death, the degree of formation of the non-open water area, the degree of death, the degree of formation, the degree of formation. The pathogenicity of the bacteria in the non-open water and non-open water areas was increased, and the pathogenicity of the bacteria was increased. In this study, the pathogenicity of R. quercivora strains was different, and the pathogenicity of bacteria in the wood was elongated. The color gamut forms the color gamut. The color gamut forms the color gamut. The color gamut forms the color gamut, the color gamut, the color gamut. In Japan, 32 strains were selected, strains 4 with enzyme activity were selected, seedlings were selected, adult branches were inoculated at 1 point, and the mortality was calculated by cross-section. It is necessary to calculate the amount of bacteria in the raw material at 1: 00. The results showed that the death rate of enzyme activity among strains was cut off in non-open water, and the bacteria were deliberately isolated. The mortality rate of bacteria and enzyme activity in non-open waters, the growth of bacteria among strains, the mortality rate of bacteria in non-open waters, the mortality rate of bacteria, the mortality rate, the mortality, the The pathogenicity of the above bacteria, the pathogenicity of the bacteria, the activity of the enzyme, and the low activity of the pathogenicity of the bacteria, the pathogenicity of the bacteria, the activity of enzymes and the activity of enzymes. The pathogenicity of strains was different, and the degree of strain extension of bacteria in the wood was different from that of bacteria. Now I am going to study the first part of the book, and the doctor will take it from me.