Control of cellular microenvironments and analysis of cellular functions by using caged culture substrates

响应细胞外刺激改变表面细胞粘附性的动态基质在基础生物学研究和组织工程应用中是有用的。本项目致力于光响应型“笼式培养基质”的开发和应用。通过控制氨基密度的表面功能化，我们成功地提高了光开关效率。此外，通过分析受控细胞微环境中的细胞迁移，我们发现细胞随着集群大小和孵化时间的增加而呈现聚集特征。

Dynamic substrates that change surface cell adhesiveness in response to an extracellular stimulus are useful for fundamental biological studies as well as tissue engineering applications. This project focused on the development and applications of photo-responsive “caged culture substrates”. By functionalization of a surface with the controlled amino group density, we succeeded in improving the photoswitching efficiency. Furthermore, by analyzing cell migration in controlled cellular microenvironments, we found that the cells achieve collective characteristics as increasing cluster size as well as incubation time.