Construction of sterilization technique based on biocidalradicals-producing photoreaction for the progression of regenerative medicine

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The antimicrobial ability of near-ultraviolet irradiation was investigated by a bactericidal assay in a cell buffer solution with a light-emitting diode. At 30 J/cm^<2>of accumulated near-ultraviolet irradiation, survivors of Escherichia coli, Staphylococcus aureus and Saccharomyces cerevisiae reduced to 1/1000, 1/100 and 1/100 of their initial survivors, respectively. The effect of near-ultraviolet irradiation on specific growth rate of human neonate dermal fibroblasts was determined after addition of morin as an antioxidant to cell cultivation broth, followed by irradiation. Morin had the highest inhibition efficacy against generating reactive oxygen species in cells. As a result, the addition of morin increased the specific growth rate by 34% at 5.0 J/cmcm^<2>irradiation.