Development of Interfacial Functional Membrane for Continuous Protein Refolding

蛋白质由于其高度的底物特异性和反应性而具有广泛的应用领域。该蛋白在大肠杆菌中表达后，通常必须经过重折叠才能恢复其活性。杆菌为了实现蛋白质的大规模生产，蛋白质的连续折叠方法将是今后的研究重点。为了积极利用未折叠蛋白质和复性介质之间的界面，这是特定的连续方法，开发和使用的功能聚合物膜，可以帮助蛋白质在界面中的复性已被首次提出。在本研究中，具体地，提出了如何通过等离子体接枝聚合方法制造这样的膜的方法，并探索了蛋白质重折叠所需的膜性能。

Protein has wide application area due to its high substrate specificity and reactivity. The protein often must be refolded to regain its activity after the expression in E. coli. For the mass production of the protein, continuous protein folding method will be focused in the near future. To positively utilize the interface between unfolded protein and refolding media, which is specific for the continuous method, the development and the usage of functional polymeric membranes that can help protein refolding in the interface has been proposed for the first time. In the present study, specifically, the method how to fabricate such a membrane via plasma graft polymerization methodology is proposed and the membrane performance that is required for the protein refolding has been explored.