Inactivation mechanisms of voltage-gated Ca channels by CaィイD12+ィエD1 in smooth muscle cells.

Voltage-gated Ca channels in smooth muscle cells are the most important pathway for allowing Ca D12+イエD1 to flow down a concentration gradient into the cell。在目前的研究中，我们探索了电压门的钙通道使用全细胞贴片钳技术的不激活机制。所取得的结果的概要如下：1. Carbachol （在Muscarinic receptors中表演）或组胺（在H1组胺receptors中表演）抑制的电压门Ca信道电流（I-D2Ca-D2）引起的脉冲在双氧标记中被抑制，一个初始瞬态分量跟随的分量。磷脂质代谢在I-D2Ca-D2的双羟基抑制中隐含的可能性被研究了使用磷脂质抑制剂的可能性，例如Wortmannin和D 609。The results show that phospholipase C is involved in the transient component of IイイD2CaイエD2 suppression， and phospholipase C or D in the sustained component of IイD2CaイエD2 suppression. 2。如果要看细胞基酮，那么作为活性素微膜和微管，就会发生在由碳水化合物诱导的I-D2Ca-D2的双重抑制中，细胞基酮Depolymerizers和聚合物对碳水化合物行动的影响。The results sugget that the bipbasic suppression of IイイD2 CaイエD2 is mediated by microtubule polymerization. 3。其磷酸化在I-D2 Ca-D2的双重抑制中被揭示，研究了使用蛋白激酶A、蛋白激酶G、蛋白激酶C和钙调蛋白依赖肌红蛋白光链激酶的抑制剂和激活剂。The result suggest that phosphorylation process is not involved in the biphasic suppression of IイイD2CaイエD2。The above results have been published as 2 papers in the Britishi Journal of Pharmacology。肌肉受体刺激如何使微管发生聚合物，以及微管聚合物抑制电压--门控钙通道活动将被测试。

Voltage-gated Ca channels in smooth muscle cells are the most important pathway for allowing CaィイD12+ィエD1 to flow down a concentration gradient into the cell. In the present study, we explored the inactivation mechanisms of voltage-gated Ca channels using the whole-cell patch clamp technique. Outlines of the obtained results are as follows.1. Carbachol (acting at muscarinic receptors) or histamine (acting at H1 histamine receptors) suppressed voltage-gated Ca channel currents (IィイD2CaィエD2) evoked by depolarizing pulses in a biphasic manner, an initial transient component followed by a sustained component. The possibility that phospholipid metabolisms are implicated in the biphasic suppression of IィイD2CaィエD2 was examined using inhibitors of phospholipases, such as wortmannin and D609. The results show that phospholipase C is involved in the transient component of IィイD2CaィエD2 suppression, and phospholipase C or D in the sustained component of IィイD2CaィエD2 suppression.2. To see if cytoskeletons, such as actin microfilaments and microtubules, are involved in the biphasic suppression of IィイD2CaィエD2 induced by carbachol, effects of cytoskeletal depolymerizers and polymerizers on the carbachol action were investigated. The results sugget that the bipbasic suppression of IィイD2CaィエD2 is mediated by microtubule polymerization.3. The possibility thet phosphorylation of Ca channels is implicated in the biphasic suppression of IィイD2CaィエD2 was examined using inhibitors and activators of protein kinase A, protein kinase G, protein kinase C and calmodulin dependent myosin light chain kinase. The results suggest that phosphorylation process is not involved in the biphasic suppression of IィイD2CaィエD2.The above results have been published as 2 papers in the Britishi Journal of Pharmacology. How muscarinic receptor stimulation polymerizes microtubules, and how microtubule polymerization suppress voltage-gated Ca channel activity will be examined.