Study of calcitonin (CT) family in an amphioxus, Branchiostoma floridae : Coevolution among CT peptide, CT receptor, and receptor activity- modifying protein.

文昌鱼、佛罗里达文昌鱼降钙素 (CT) 家族的研究：CT 肽、CT 受体和受体活性修饰蛋白之间的共同进化。

• 批准号: 23770069
• 负责人: SEKIGUCHI Toshio
• 金额: $ 2.91万
• 依托单位: Kanazawa University (2012)Suntory Foundation for Life Sciences (2011)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23770069/
• 关键词: Calcitonin; Calcitonin receptor; RAMP; ナメクジウオ; Calcitonin 受容体; 受容体修飾蛋白; 分子進化; 比較内分泌学

Calcitonin (CT) family shows high molecular and functional diversification in vertebrates. To elucidate the origin of CT family, CT peptide, CT receptor (CTR), and receptor activity-modifying protein (RAMP) were identified from an amphioxus, Branchiostoma floridae, which is close animal of vertebrates. Furthermore, analysis ofCTR and RAMP expressed in mammalian cell line demonstrated that co-expression of CTR and RAMP is required for ligand activity of CT peptide, and that RAMP is necessary for cell-surface translocation of CTR. These results let us conclusion that CT peptide, CTR, and RAMP have already existed in the common ancestor between vertebrates and amphioxus.

降钙素(CT)家族在脊椎动物中表现出高分子和功能的多样性。为了阐明CT家族的起源，从文昌鱼(Branchiostoma Floridae)中鉴定了CT肽、CT受体(CTR)和受体活性修饰蛋白(RAMP)。此外，对CTR和RAMP在哺乳动物细胞中表达的分析表明，CTR和RAMP的共表达是CT多肽的配体活性所必需的，而RAMP是CTR细胞表面转位所必需的。这些结果表明，文昌鱼和脊椎动物的共同祖先中已经存在CT肽、CTR和RAMP。

项目成果

脊椎動物祖先型生物ホヤはハイブリッド型のToll様受容体を持つ

脊椎动物的祖先海鞘具有一种混合型 Toll 样受体。

• 发表时间: 2011
• 期刊: エンドトキシン・自然免疫研究
• 作者: 佐々木 尚子;関口 俊男;楠本 正一;佐竹 炎
• 通讯作者: 佐竹 炎

Peptidomic Analysis of the Central Nervous System of the Protochordate, Ciona intestinalis: Homologs and Prototypes of Vertebrate Peptides and Novel Peptides

• DOI: 10.1210/en.2010-1348
• 发表时间: 2011-06-01
• 期刊: ENDOCRINOLOGY
• 影响因子: 4.8
• 作者: Kawada, Tsuyoshi;Ogasawara, Michio;Satake, Honoo
• 通讯作者: Satake, Honoo

Cloning of two members of the calcitonin-family receptors from stingray, Dasyatis akajei Possible physiological roles of the calcitonin family in osmoregulation

从黄貂鱼 Dasyatis akajei 中克隆降钙素家族受体的两个成员 降钙素家族在渗透压调节中的可能生理作用

• DOI: 10.1016/j.gene.2012.03.042
• 发表时间: 2012
• 期刊: Gene
• 影响因子: 3.5
• 作者: Suzuki N;Sekiguchi T;Satake H;Kato K;Nishiyama Y;Takahashi H;et al
• 通讯作者: et al

Polychlorinated biphenyl (118) activates osteoclasts and induces bone resorption in goldfish.

• DOI: 10.1007/s11356-012-1347-5
• 发表时间: 2014-05
• 期刊: Environmental science and pollution research international
• 作者: Yachiguchi K;Matsumoto N;Haga Y;Suzuki M;Matsumura C;Tsurukawa M;Okuno T;Nakano T;Kawabe K;Kitamura K;Toriba A;Hayakawa K;Chowdhury VS;Endo M;Chiba A;Sekiguchi T;Nakano M;Tabuchi Y;Kondo T;Wada S;Mishima H;Hattori A;Suzuki N
• 通讯作者: Suzuki N

カタユウレイボヤ Toll-like receptor リガンドにより誘起される生体防御機構の解析

Ciona Toll样受体配体诱导的生物防御机制分析

• 发表时间: 2011
• 作者: 関口俊男;佐々木尚子;楠本正一;佐竹炎
• 通讯作者: 佐竹炎