MCAA: Development of Laboratory Skills in Molecular Biology to Examine Patterns of Calcitonin and Calcitonin Receptor Gene Expression in Cartilaginous Fishes.

MCAA:发展分子生物学实验室技能,以检查软骨鱼中降钙素和降钙素受体基因表达的模式。

基本信息

  • 批准号:
    0416454
  • 负责人:
  • 金额:
    --
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Standard Grant
  • 财政年份:
    2004
  • 资助国家:
    美国
  • 起止时间:
    2004-07-15 至 2005-12-31
  • 项目状态:
    已结题

项目摘要

MCAA: Development of laboratory skills in molecular biology to examine patterns of calcitonin and calcitonin receptor gene expression in cartilaginous fishes. Principal Investigator: James GelsleichterThe hormone calcitonin is best known for its ability to reduce bone demineralization in mammals and, as a result of this action, it is regularly administered to patients experiencing osteoporosis, Paget's disease, or similar skeletal maladies. However, despite its therapeutic value for skeletal diseases, a growing body of evidence has suggested that the true physiological function of calcitonin may be to regulate certain aspects of vertebrate reproduction. Recently, the PI has demonstrated similar evidence of a role for calcitonin in regulating events during pregnancy in live-bearing sharks and rays, the oldest living vertebrate group known to possess the ultimobranchial gland, the site of calcitonin production. In this project, the PI will receive new training in molecular biology, which will clarify the roles of calcitonin in elasmobranch reproduction by examining patterns of calcitonin and calcitonin receptor gene expression in gravid female rays and their offspring. Following completion of an intensive, 3-week training course in molecular biology, protein chemistry, and bioinformatics at the University of Florida's Interdisciplinary Center for Biotechnology Research, the PI and two student interns will utilize RT-PCR, Northern blot analysis, and in situ hybridization to determine: 1) if increases in ultimobranchial gland activity previously shown to occur during pregnancy in this species reflect changes in calcitonin production; 2) if tissues other than the ultimobranchial gland synthesize calcitonin; and 3) where calcitonin receptors are expressed and if these expression patterns change during reproduction. By obtaining this information, this project will significantly advance knowledge regarding both reproductive physiology and endocrine function in the cartilaginous fishes and the role of calcitonin in vertebrates. Furthermore, both the training and laboratory instrumentation supported by this grant will enhance the infrastructure for research and student training opportunities in the PI's laboratory. The PI is a Native Pacific Islander researcher who makes significant contributions to both his field of study and undergraduate science education, especially that for underserved minority groups.
MCAA:发展分子生物学实验室技能,以检测软骨鱼类降钙素和降钙素受体基因表达模式。 主要研究者:降血钙素因其在哺乳动物中减少骨脱矿作用的能力而广为人知,由于这种作用,降血钙素被定期用于患有骨质疏松症、佩吉特病或类似骨骼疾病的患者。 然而,尽管降钙素对骨骼疾病具有治疗价值,但越来越多的证据表明,降钙素的真正生理功能可能是调节脊椎动物生殖的某些方面。 最近,PI已经证明了降钙素在怀孕期间调节活轴承鲨鱼和射线事件中的作用的类似证据,已知最古老的活脊椎动物群拥有后鳃腺,降钙素生产的网站。 在本项目中,PI将接受分子生物学方面的新培训,通过检查妊娠雌性鳐及其后代中降钙素和降钙素受体基因表达的模式,阐明降钙素在板鳃亚纲动物生殖中的作用。 在佛罗里达大学跨学科生物技术研究中心完成为期3周的分子生物学、蛋白质化学和生物信息学强化培训课程后,PI和两名实习生将利用RT-PCR、北方印迹分析和原位杂交确定:1)如果增加后鳃腺活动先前显示发生在怀孕期间在这个物种反映降钙素生产的变化; 2)后鳃腺以外的组织是否合成降钙素; 3)降钙素受体在哪里表达,以及这些表达模式是否在生殖过程中发生变化。 通过获得这些信息,该项目将大大推进有关软骨鱼类的生殖生理和内分泌功能以及降钙素在脊椎动物中的作用的知识。 此外,培训和实验室仪器由这笔赠款支持将加强研究和学生在PI的实验室培训机会的基础设施。 PI是一个本土的太平洋岛民研究人员谁使他的研究领域和本科科学教育的重大贡献,特别是对服务不足的少数群体。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

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James Gelsleichter其他文献

Associations between total mercury, trace minerals, and blood health markers in Northwest Atlantic white sharks (Carcharodon carcharias).
西北大西洋白鲨(Carcharodon carcharias)的总汞、微量矿物质和血液健康标志物之间的关联。
  • DOI:
    10.1016/j.marpolbul.2023.115533
  • 发表时间:
    2023
  • 期刊:
  • 影响因子:
    5.8
  • 作者:
    Lisa M Crawford;James Gelsleichter;Alisa L. Newton;L. Hoopes;Cheng;Nicholas S. Fisher;Douglas H. Adams;Maeva Giraudo;Anne E. McElroy
  • 通讯作者:
    Anne E. McElroy
Female sperm storage in the bonnethead <em>Sphyrna tiburo</em> oviducal gland: Immunolocalization of steroid hormone receptors in sperm storage tubules
  • DOI:
    10.1016/j.ygcen.2021.113827
  • 发表时间:
    2021-09-01
  • 期刊:
  • 影响因子:
  • 作者:
    Melissa Gonzalez De Acevedo;James Gelsleichter
  • 通讯作者:
    James Gelsleichter
Associations between total mercury, trace minerals, and blood health markers in Northwest Atlantic white sharks (emCarcharodon carcharias/em)
西北大西洋白鲨(Carcharodon carcharias)中总汞、微量矿物质与血液健康标志物之间的关联
  • DOI:
    10.1016/j.marpolbul.2023.115533
  • 发表时间:
    2023-10-01
  • 期刊:
  • 影响因子:
    4.900
  • 作者:
    Lisa M. Crawford;James Gelsleichter;Alisa L. Newton;Lisa A. Hoopes;Cheng-Shiuan Lee;Nicholas S. Fisher;Douglas H. Adams;Maeva Giraudo;Anne E. McElroy
  • 通讯作者:
    Anne E. McElroy
Associations between total mercury, trace minerals, and blood health markers in Northwest Atlantic white sharks (emCarcharodon carcharias/em)
西北大西洋白鲨(Carcharodon carcharias)中总汞、微量矿物质与血液健康标志物之间的关联
  • DOI:
    10.1016/j.marpolbul.2023.115533
  • 发表时间:
    2023-10-01
  • 期刊:
  • 影响因子:
    4.900
  • 作者:
    Lisa M. Crawford;James Gelsleichter;Alisa L. Newton;Lisa A. Hoopes;Cheng-Shiuan Lee;Nicholas S. Fisher;Douglas H. Adams;Maeva Giraudo;Anne E. McElroy
  • 通讯作者:
    Anne E. McElroy
Morphological and histological changes inthe genital ducts ofthe male Atlantic stingray, Dasyatis sabina, during the seasonal reproductive cycle
雄性大西洋黄貂鱼 (Dasyatis sabina) 生殖管在季节性繁殖周期中的形态和组织学变化
  • DOI:
  • 发表时间:
    2003
  • 期刊:
  • 影响因子:
    2.9
  • 作者:
    A. Piercy;James Gelsleichter;F. F. Snelson
  • 通讯作者:
    F. F. Snelson

James Gelsleichter的其他文献

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{{ truncateString('James Gelsleichter', 18)}}的其他基金

Development of a Strategic Plan for the William C. Webb Coastal Research Station
制定威廉·C·韦伯沿海研究站战略计划
  • 批准号:
    1820956
  • 财政年份:
    2018
  • 资助金额:
    --
  • 项目类别:
    Standard Grant
REU Site: Research Experiences for Undergraduates in Coastal Biology on Florida's First Coast.
REU 网站:佛罗里达州第一海岸沿海生物学本科生的研究经验。
  • 批准号:
    1156659
  • 财政年份:
    2012
  • 资助金额:
    --
  • 项目类别:
    Continuing Grant
Research Experiences in Estuarine Science for Undergraduates at Mote Marine Laboratory: Developing a New Generation of Leaders in Ocean Sciences
莫特海洋实验室本科生河口科学研究经验:培养新一代海洋科学领军人物
  • 批准号:
    0453955
  • 财政年份:
    2005
  • 资助金额:
    --
  • 项目类别:
    Continuing Grant
Research Experiences in Estuarine Science for Undergraduates at Mote Marine Laboratory, with Special Focus on Native Pacific Islanders
莫特海洋实验室本科生河口科学研究经验,特别关注太平洋原住民
  • 批准号:
    0139392
  • 财政年份:
    2002
  • 资助金额:
    --
  • 项目类别:
    Continuing Grant
MRPG: Investigations on the Role of Calcitonin in Reproduction and Development of the Atlantic Stingray
MRPG:降钙素在大西洋黄貂鱼繁殖和发育中作用的研究
  • 批准号:
    9986070
  • 财政年份:
    2000
  • 资助金额:
    --
  • 项目类别:
    Standard Grant
Minority Postdoctoral Research Fellowships and Supporting Activities Program Travel Award
少数民族博士后研究奖学金及配套活动计划旅游奖
  • 批准号:
    9713162
  • 财政年份:
    1997
  • 资助金额:
    --
  • 项目类别:
    Fellowship Award

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