Structural analysis of the Rab and SNARE localization

Rab 和 SNARE 定位的结构分析

• 批准号: 23770107
• 负责人: ITO Sakurako
• 金额: $ 2.91万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23770107/
• 关键词: X線結晶構造解析; タンパク質; 生体膜; 立体構造解析; 膜融合; X線結晶解析; 小胞輸送

In eukaryotic cells, Rab and SNARE regulate the fusion of membrane compartments. Rabs are inseted into membranes via the prenylated moieties which are post-translationally attached. A membrane protein called GDF is proposed to catalyze the membrane insertion of Rab. As GDF and VAMP2, one of the SNARE protein, is shown to interact with each other, we are trying to reveal the structural basis of GDF-SNARE cooperation. So far, we have succeeded in the crystallization of GDF, and now trying to improve thediffraction quality of crystals. Also, we are trying to prepare GDF-VAMP2 complex.

在真核细胞中，Rab和SNARE调节膜隔室的融合。Rabs通过后连接的异戊二烯化部分插入膜中。一种被称为GDF的膜蛋白被提出来催化Rab的膜插入。由于GDF和SNARE蛋白之一的VAMP 2相互作用，我们试图揭示GDF-SNARE合作的结构基础。到目前为止，我们已经成功地结晶了GDF，现在正在努力提高晶体的衍射质量。此外，我们正在尝试制备GDF-VAMP 2复合物。

项目成果

Get3 による翻訳後膜埋込み機構の構造学的基盤

Get3翻译后膜嵌入机制的结构基础

• 发表时间: 2012
• 作者: Saito;N.;山形敦史
• 通讯作者: 山形敦史

Crystallographic and mutational studies on the tRNA thiouridine synthetase TtuA

• DOI: 10.1002/prot.24273
• 发表时间: 2013-07-01
• 期刊: PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS
• 影响因子: 2.9
• 作者: Nakagawa, Hirofumi;Kuratani, Mitsuo;Yokoyama, Shigeyuki
• 通讯作者: Yokoyama, Shigeyuki