Studies on the direction of mutations in relation with gene expression level

突变方向与基因表达水平相关的研究

• 批准号: 23780008
• 负责人: HASE Yoshihiro
• 金额: $ 1.75万
• 依托单位: Japan Atomic Energy Agency
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23780008/
• 关键词: 植物育種; 突然変異

This study focused on the direction of mutation in order to improve the efficiency of plant mutation breeding. The expression level of Arabidopsis genes involved in anthocyanin biosynthesis was elevated by sucrose treatment. The frequency of pigment-less mutants induced by ion beam irradiation was higher in sucrose-treated seedlings than in non-treated seedlings. This suggests the possibility that the highly-expressed genes are relatively mutable.

本研究着重于诱变方向的研究，以期提高植物诱变育种的效率。蔗糖处理提高了拟南芥花色素苷合成相关基因的表达水平。离子束辐照诱导的色素缺失突变体的频率在蔗糖处理的幼苗中高于未处理的幼苗。这表明高表达基因相对可变的可能性。

项目成果

Mutational effects of carbon ions near the range end and development of an efficient mutagenesis technique using ion beams

碳离子在范围末端附近的突变效应以及使用离子束的有效诱变技术的开发

• 发表时间: 2013
• 期刊: JAEA-Review
• 作者: Yoshihiro Hase;Ryouhei Yoshihara;Shigeki Nozawa;Issay Narumi
• 通讯作者: Issay Narumi

飛程末端に近い炭素イオンの変異誘発効果と効率的な変異誘発技術の開発

碳离子近端诱变效应及高效诱变技术开发

• 发表时间: 2012
• 作者: Yoshihiro Hase;Ryouhei Yoshihara;Shigeki Nozawa;Issay Narumi;Yoshihiro Hase;長谷純宏
• 通讯作者: 長谷純宏