Understanding the sex-determining processes and the molecular mechanism of reproductive manipulations in insects

当转染到雄性家蚕细胞系（M1）时，雌性化沃尔巴克氏体内生共生体来源于中国产真核线虫（Eurema mandarina），影响性别决定基因双性（dsx）的性别特异性剪接。感染后2 ~ 4周，dsx开始出现雄性和雌性的剪接产物。初步的大阵列分析结果显示了受雌性化沃尔巴克氏体影响的数十个候选基因，目前正在进一步分析中。雌性化沃尔巴克氏体成功转染到雄性果蝇细胞系（S2）中，但dsx的剪接模式保持不变。

When transinfected into a male Bombyx mori cell line (M1), feminizing Wolbachia endosymbiont derived from Eurema mandarina affected sex-specific splicing of the sex-determining gene doublesex (dsx). Two to four weeks after transinfection, dsx begins to exhibit splicing products of both male and female. Preliminary results of the macroarray analyses demonstrated dozens of candidate genes affected by the feminizing Wolbachia, which are currently under further analyses. The feminizing Wolbachia was successfully transinfected into a male Drosophila cell line (S2), but the splicing pattern of dsx remained unchanged.