Role of a novel photoproduct found in sensory rhodopsin II in the function and its characterization

感觉视紫红质II （SRII）是盐杆菌中的一种疏光受体蛋白。在这项研究中，我们试图表征一种长寿命的光产物（Mlike中间体），该产物是由盐盐杆菌在连续光照下在SRII中形成的，并揭示了它的生理作用。通过羟胺（一种水溶性试剂）的实验，观察了蛋白质在m态时内部的亲水性环境变化。此外，我们还研究了位于视网膜附近的两个氨基酸残基（Asp103和Ser201）突变对视网膜趋光性的影响。

Sensory rhodopsin II (SRII) is a photophobic receptor protein in halobacteria. In this study, we attempted to characterize a long-lived photoproduct (Mlike intermediate) that was formed in SRII from Halobacterium salinarum under continuous illumination and reveal the physiological role of it. Through the experiment using hydroxylamine, a water-soluble reagent, the hydrophilic environmental change in the protein interior at Mlike state was observed. In addition, the effects of the mutation of two amino acid residues (Asp103 and Ser201) located in the vicinity of the retinal on the phototaxis were investigated.