In vivo analysis of the Hippo signaling pathway during small fish embryogenesis.

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To monitor the cell-cycle progression in the developing medaka embryos, we adapted the fluorescent ubiquitylation-based cell cycle indicator (Fucci) system. We observed that proliferating cells were concentrated in several organs, including the retina and brain during later morphogenesis. Next, we investigated whether the Hippo signaling pathway affects later morphogenesis during early zebrafish development. Knockdown of the Mst2, one of the major components of the Hippo pathway, induced morphological defects in the zebrafish eye, implying that the Hippo pathway plays a critical role inthe retinal cell proliferation and differentiation.