Molecular mechanisms of zinc-induced epigenetic changes in chromatinof MTF-1 target gene promoters

锌是一种必需的微量营养素。严格控制细胞内锌浓度至关重要。在哺乳动物中，金属硫蛋白（MT）是一种小分子金属结合蛋白，在锌的稳态中起重要作用。小鼠MT-I基因转录受金属反应元件结合转录因子-I（MTF-I）调节，其被锌募集到启动子。以前我们表明，MT-I启动子的染色质结构的改变与增强的转录激活有关。在这里，我们发现染色质重塑因子Brm和Brg 1不参与基因表达。

Zinc is an essential micronutrient. It is critically important to control intracellular zinc concentrations tightly. In mammals, metallothionein (MT), a small metal-binding protein, plays important roles in zinc homeostasis. Mouse MT-I gene transcription is regulated by metal response element-binding transcription factor-l (MTF-l), which is recruited to the promoter by zinc. Previously we showed that alterations in the chromatin structure of the MT-I promoter associated with enhanced transcriptional activation. Here, we showed that chromatin remodeling factor Brm and Brg1 was not involved in the gene expression.