Genomic profiling of renal cell carcinoma in patients with end-stage renal disease

终末期肾病患者肾细胞癌的基因组分析

• 批准号: 23790408
• 负责人: INOUE Toru
• 金额: $ 2.33万
• 依托单位: Oita University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23790408/
• 关键词: 分子病理; 終末期腎癌; CGHアレイ; 終末期腎; 腎癌; ゲノム異常

The purpose of this study was to determine the genomic profile of renal cell carcinoma in end-stage renal disease (RCC-ESRD) by analysis of genomic copy number aberrations (CNAs). Seventy-nine tumor samples from 63 patients with RCC-ESRD were analyzed by array comparative genomic hybridization (array CGH) using the Agil ent Whole Human Genome 4×44K Oligo Micro Array. Unsupervised hierarchical clustering analysis revealed that the 63 cases were divisible into two groups: clusters A and B. Cluster A comprised mainly clear cell RCCs (CCRCCs), whereas cluster B comprised mainly papillary RCCs (PRCCs), acquired cystic disease (ACD)-associated RCCs and clear cell papillary RCCs. Analysis of the averaged frequencies revealed that the genomic profiles of clusters A and B resembled those of sporadic CCRCC and sporadic papillary RCC (PRCC), respectively. Although, on the basis of histopathology, it has been proposed that ACD-associated RCC, clear cell papillary RCC and PRCC-ESRD are distinct subtypes, our present data reveal that their genomic profiles categorized as cluster B resemble one another. Furthermore, genomic profiles of PRCC, ACD-associated RCC and clear cell papillary RCC admixed in one tissue tended to resemble one another. On the basis of genomic profiling of RCC-ESRD, we conclude that the molecular pathogenesis of CCRCC-ESRD resembles that of sporadic CCRCC. Although various histologic subtypes of non-CCRCC-ESRD have been proposed, their genomic profiles resemble those of sporadic PRCC, suggesting that the molecular pathogenesis of non-CCRCC-ESRD might be related to that of sporadic PRCC.

这项研究的目的是通过分析基因组拷贝数畸变（CNAS）来确定末期肾脏疾病（RCC-ESRD）中肾细胞癌的基因组谱。使用阵列比较基因组杂交（阵列CGH）分析了来自63例RCC-ESRD患者的79个肿瘤样品，并使用全人类基因组4×44K寡质阵列分析。无监督的层次聚类分析表明，63例可将两组分为两组：簇A和B。聚类A完整的细胞RCC（CCRCCS），而B群集B完成了主要的乳头状RCC（PRCCS），是囊肿疾病（ACD）同时化的RCCS和Clear Papillary RCCS。对平均频率的分析表明，簇A和B的基因组谱分别类似于零星的CCRCC和零星乳头状乳头RCC（PRCC）。尽管基于组织病理学，已经提出，与ACD相关的RCC，透明细胞乳头RCC和PRCC-ESRD是不同的亚型，但我们的目前数据表明，它们的基因组谱分为群体B彼此相似。此外，在一种组织中混合了PRCC，ACD相关的RCC和清除细胞乳头RCC的基因组谱，往往相似。根据RCC-ESRD的基因组分析，我们包含CCRCC-ESRD的分子发病机理类似于Spuradic CCRCC。尽管已经提出了非CCRCC-ESRD的各种组织学亚型，但它们的基因组谱类似于Spuradic PRCC的基因组，这表明非CCRCC-ESRD的分子发病机理可能与Spuradic PRCC有关。

项目成果

Array CGH analysis of renal cell carcinomas in patients with end-stage

终末期肾细胞癌的阵列 CGH 分析

• 发表时间: 2011
• 作者: Komohara Y;Hasita H;Ohnishi K;Fujiwara Y;Suzu S;Eto M;Takeya M.;井上 享
• 通讯作者: 井上 享

Genomic profiling of renal cell carcinoma in patients with end-stage renal disease

• DOI: 10.1111/j.1349-7006.2011.02176.x
• 发表时间: 2012-03-01
• 期刊: CANCER SCIENCE
• 影响因子: 5.7
• 作者: Inoue, Toru;Matsuura, Keiko;Moriyama, Masatsugu
• 通讯作者: Moriyama, Masatsugu