Genomic profiling of renal cell carcinoma in patients with end-stage renal disease

终末期肾病患者肾细胞癌的基因组分析

• 批准号: 23790408
• 负责人: INOUE Toru
• 金额: $ 2.33万
• 依托单位: Oita University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23790408/
• 关键词: 分子病理; 終末期腎癌; CGHアレイ; 終末期腎; 腎癌; ゲノム異常

The purpose of this study was to determine the genomic profile of renal cell carcinoma in end-stage renal disease (RCC-ESRD) by analysis of genomic copy number aberrations (CNAs). Seventy-nine tumor samples from 63 patients with RCC-ESRD were analyzed by array comparative genomic hybridization (array CGH) using the Agil ent Whole Human Genome 4×44K Oligo Micro Array. Unsupervised hierarchical clustering analysis revealed that the 63 cases were divisible into two groups: clusters A and B. Cluster A comprised mainly clear cell RCCs (CCRCCs), whereas cluster B comprised mainly papillary RCCs (PRCCs), acquired cystic disease (ACD)-associated RCCs and clear cell papillary RCCs. Analysis of the averaged frequencies revealed that the genomic profiles of clusters A and B resembled those of sporadic CCRCC and sporadic papillary RCC (PRCC), respectively. Although, on the basis of histopathology, it has been proposed that ACD-associated RCC, clear cell papillary RCC and PRCC-ESRD are distinct subtypes, our present data reveal that their genomic profiles categorized as cluster B resemble one another. Furthermore, genomic profiles of PRCC, ACD-associated RCC and clear cell papillary RCC admixed in one tissue tended to resemble one another. On the basis of genomic profiling of RCC-ESRD, we conclude that the molecular pathogenesis of CCRCC-ESRD resembles that of sporadic CCRCC. Although various histologic subtypes of non-CCRCC-ESRD have been proposed, their genomic profiles resemble those of sporadic PRCC, suggesting that the molecular pathogenesis of non-CCRCC-ESRD might be related to that of sporadic PRCC.

本研究的目的是通过分析基因组拷贝数异常(CNA)来确定终末期肾病肾细胞癌(RCC-ESRD)的基因组图谱。应用Agil全人类基因组4×44K寡核苷酸微阵列对63例肾癌-终末期肾病患者的79例肿瘤标本进行阵列比较基因组杂交(阵列CGH)分析。非监督系统聚类分析显示，63例患者可分为A、B两组，A组主要为透明细胞RCC(CcRCC)，B组主要为乳头状RCC、获得性囊性疾病相关RCC和透明细胞乳头状RCC。对平均频率的分析表明，A和B簇的基因组图谱分别与散发性肾癌和散发性乳头状肾癌相似。尽管在组织病理学的基础上，ACD相关性RCC、透明细胞乳头状RCC和PRCC-ESRD是不同的亚型，但我们目前的数据显示，它们被归类为B簇的基因组图谱彼此相似。此外，混合在同一组织中的PRCC、ACD相关性RCC和透明细胞乳头状RCC的基因组图谱趋向于彼此相似。根据RCC-ESRD的基因组图谱，我们认为ccRCC-ESRD的分子发病机制类似于散发性ccRCC。尽管非ccRCC-ESRD的组织学亚型不同，但它们的基因组图谱与散发性PRCC相似，提示非ccRCC-ESRD的分子发病机制可能与散发性PRCC有关。

项目成果

Array CGH analysis of renal cell carcinomas in patients with end-stage

终末期肾细胞癌的阵列 CGH 分析

• 发表时间: 2011
• 作者: Komohara Y;Hasita H;Ohnishi K;Fujiwara Y;Suzu S;Eto M;Takeya M.;井上 享
• 通讯作者: 井上 享

Genomic profiling of renal cell carcinoma in patients with end-stage renal disease

• DOI: 10.1111/j.1349-7006.2011.02176.x
• 发表时间: 2012-03-01
• 期刊: CANCER SCIENCE
• 影响因子: 5.7
• 作者: Inoue, Toru;Matsuura, Keiko;Moriyama, Masatsugu
• 通讯作者: Moriyama, Masatsugu