Identification of functional domains of influenza C virus CM2 protein involved in viral replication

丙型流感病毒CM2蛋白参与基因组包装和脱壳。为了阐明CM2参与这些功能的结构域，我们在CM2胞质尾区引入了一系列缺失和取代突变。我们对这些突变体进行了分析，得到以下结果. CM2蛋白C端53个氨基酸不是其转运到细胞表面所必需的. CM2胞质尾区的特异性氨基酸序列是其胞内转运所必需的.细胞质尾区是稳定寡聚化（形成二聚体和四聚体）所必需的。

Influenza C virus CM2 protein is involved in genome packaging and uncoating. To clarify the domains of CM2 participating in these functions, we introduced a series of deletion and substitution mutations in the CM2 cytoplasmic tail. We analyzed these mutants and obtained the following results.1. C-terminal 53 amino acids of CM2 protein are not essential for its transport to the cell surface.2. The specific amino acid sequence of cytoplasmic tail of CM2 is required for its intracellular transport.3. The cytoplasmic tail is required for stable oligomerization (formation of a dimer and a tetramer).