Identification of functional domains of influenza C virus CM2 protein involved in viral replication

丙型流感病毒CM2蛋白参与了基因组的包装和剥离。为了阐明参与这些功能的CM2结构域，我们在CM2细胞质尾部引入了一系列缺失和替代突变。我们对这些突变体进行了分析，得到了以下结果：CM2蛋白的c端53个氨基酸对于其转运到细胞表面并不是必需的。CM2胞质尾部特定的氨基酸序列是其胞内转运所必需的。细胞质尾部是稳定寡聚化（二聚体和四聚体的形成）所必需的。

Influenza C virus CM2 protein is involved in genome packaging and uncoating. To clarify the domains of CM2 participating in these functions, we introduced a series of deletion and substitution mutations in the CM2 cytoplasmic tail. We analyzed these mutants and obtained the following results.1. C-terminal 53 amino acids of CM2 protein are not essential for its transport to the cell surface.2. The specific amino acid sequence of cytoplasmic tail of CM2 is required for its intracellular transport.3. The cytoplasmic tail is required for stable oligomerization (formation of a dimer and a tetramer).