Assessment of the combined effect of chemicals on the ion channels of freshwater fish.

评估化学品对淡水鱼离子通道的综合影响。

• 批准号: 23790677
• 负责人: SHIMIZU Hiroyasu
• 金额: $ 0.33万
• 依托单位: Osaka Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23790677/
• 关键词: メダカ; 水生生物; 化学物質; パッチクランプ; 毒性; バイオアッセイ; スクリーニング; 水生動物; 環境毒性; イオンチャネル

The purpose of this project was to assess the effect of chemicals on the ion channels of Medaka (Oryzias latipes) cells. Epithelial cells from the gill were obtained and the patch clamp method was used to observe whole cell current. Although this observation was not difficult, the condition of the cells deteriorated after exposure to the chemicals, which affected the reliability and reproducibility of the data. Electrophysiological methods appear to be unsuitable for assessing the effect of chemicals on ion channels. Therefore, other assessment methods, such as using gene knock out Medaka, might be explored as alternatives.

本研究旨在探讨化学物质对水稻细胞离子通道的影响。取鳃上皮细胞，膜片钳法观察全细胞电流。虽然这种观察并不困难，但接触化学物质后细胞状况恶化，影响了数据的可靠性和可重复性。电生理方法似乎不适合评估化学物质对离子通道的影响。因此，可以探索其他评估方法，如基因敲除Medaka，作为替代方法。