Assessment of the combined effect of chemicals on the ion channels of freshwater fish.

本项目的目的是评估化学物质对青鳉细胞离子通道的影响。取鳃上皮细胞，采用膜片钳技术观察全细胞电流。虽然这一观察并不困难，但在接触化学品后，细胞的状况恶化，影响了数据的可靠性和再现性。电生理学方法似乎不适合评估化学品对离子通道的影响。因此，其他评估方法，如使用基因敲除青鳉，可以探索作为替代。

The purpose of this project was to assess the effect of chemicals on the ion channels of Medaka (Oryzias latipes) cells. Epithelial cells from the gill were obtained and the patch clamp method was used to observe whole cell current. Although this observation was not difficult, the condition of the cells deteriorated after exposure to the chemicals, which affected the reliability and reproducibility of the data. Electrophysiological methods appear to be unsuitable for assessing the effect of chemicals on ion channels. Therefore, other assessment methods, such as using gene knock out Medaka, might be explored as alternatives.