Study on the health effects of industrial chemical with potential hepatotoxicity

DMAc广泛应用于医药和工业化合物中。分别吸入DMAC或空白对照组(10、25、50、250ppm)14天后，发现50和250ppm的DMAC可显著提高小鼠血浆AST和ALT活性。两种基因型的组织学改变均可观察到。此外，在高浓度暴露下，细胞色素P450 2-/-也出现局灶性坏死和脂肪变性。暴露组小鼠尿中NMAC和DMAC中均检出CYP2E1-/-。此外，NMAC和DMAC的浓度高于CYP2E1/小鼠。在乙酰胺暴露组小鼠尿液中检测到了细胞色素P450 2E 1/。因此，大多数DMAC的代谢都是通过细胞色素P450-2E_1进行的，但也有可能存在其他途径。

DMAC is widely used in pharmaceutical and industrial chemical compounds. The cyp2e1+/+and cyp2e1-/- mice were exposed to inhalation of DMAC or vehicle control (10, 25, 50 and 250ppm) for 14 days., In cyp2e1+/+ and cyp2e1-/-mice, plasma AST and ALT activities were significantly increased at 50 and 250ppm of DMAC. The histological changes were observed in both genotypes. In addition, focal necrosis and fatty degeneration was also observed in the cyp2e1-/- at the high concentration exposure. cyp2e1-/- were detected in the exposed group for NMAC and DMAC in mice urine. Further, NMAC and DMAC was high concentration as compared to cyp2e1+/+ mice. cyp2e1+/+ were detected in the exposed group for acetamide in mice urine. Hence, most of the DMAC metabolism takes place via CYP2E1, but it is indicated that there is a possibility that other pathways also exist.