Study on the health effects of industrial chemical with potential hepatotoxicity

DMAC广泛用于制药和工业化学化合物。将cyp 2 e1 +/+和cyp 2 e1-/-小鼠暴露于吸入DMAC或载体对照（10、25、50和250 ppm）14天。在cyp 2 e1 +/+和cyp 2 e1-/-小鼠中，在50和250 ppm的DMAC下，血浆AST和ALT活性显著升高。两种基因型均观察到组织学改变。此外，高浓度暴露后，cyp 2 e1-/-细胞也出现局灶性坏死和脂肪变性。尿中NMAC和DMAC的阳性率分别为100%和100%。此外，与cyp 2 e1 +/+小鼠相比，NMAC和DMAC是高浓度的。乙酰胺染毒组小鼠尿中检测到CYP 2 E1 +/+。因此，大多数DMAC代谢通过CYP 2 E1进行，但这表明也可能存在其他途径。

DMAC is widely used in pharmaceutical and industrial chemical compounds. The cyp2e1+/+and cyp2e1-/- mice were exposed to inhalation of DMAC or vehicle control (10, 25, 50 and 250ppm) for 14 days., In cyp2e1+/+ and cyp2e1-/-mice, plasma AST and ALT activities were significantly increased at 50 and 250ppm of DMAC. The histological changes were observed in both genotypes. In addition, focal necrosis and fatty degeneration was also observed in the cyp2e1-/- at the high concentration exposure. cyp2e1-/- were detected in the exposed group for NMAC and DMAC in mice urine. Further, NMAC and DMAC was high concentration as compared to cyp2e1+/+ mice. cyp2e1+/+ were detected in the exposed group for acetamide in mice urine. Hence, most of the DMAC metabolism takes place via CYP2E1, but it is indicated that there is a possibility that other pathways also exist.