the role of p53 gene in maintenance of circadian rhythm

p53基因在维持昼夜节律中的作用

• 批准号: 23790838
• 负责人: HOSOYA Yumiko
• 金额: $ 2.66万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23790838/
• 关键词: 体内時計; 老化; 遺伝子; 医療福祉

Previous studies have shown that the incidence of cardiovascular events is closely associated with circadian rhythm. It is recently reported that p53 is one of the important factors modulating the development of cardiovascular disease. However, it remains unknown whether p53 affects circadian rhythm. We examined the role of p53 gene in circadian rhythm by analyzing p53 knockout mice.Daily oscillations of behavior were analyzed with actogram. P53 knockout mice demonstrated insufficient response to the light pulse and couldn't catch up the clock-resetting. Mouse embryonic fibroblast isolated from p53 knockout mice also showed the defect of p53 attenuated the oscillations of Per2 gene. Besides, microarray analysis supported the requirement of p53 gene to keep circadian rhythm intact. From these results, it is suggested that p53 can affect circadian rhythm through some feedback pathway via Per genes and may lead to modulate the incidence of vascular events.

先前的研究表明，心血管事件的发生率与昼夜节律密切相关。最近据报道，p53是调节心血管疾病发展的重要因素之一。但是，p53是否影响昼夜节律仍然未知。我们通过分析p53基因敲除小鼠研究了p53基因在昼夜节律中的作用。用actogram分析了行为的每日振荡。 p53敲除小鼠表现出对轻脉冲的反应不足，无法赶上时钟排列。从p53敲除小鼠中分离出的小鼠胚胎成纤维细胞还显示p53的缺陷减弱了PER2基因的振荡。此外，微阵列分析还支持p53基因保持昼夜节律完整的要求。从这些结果中，建议p53可以通过每个基因通过一些反馈途径影响昼夜节律，并可能导致调节血管事件的发生率。

项目成果

p5 gene can modulate the incidence of vascular events through its effect on circadian rhythm

p5基因可以通过影响昼夜节律来调节血管事件的发生率