Demonstration of Hes-1oscillation in progenitors and analysis of its effect on differentiation in hematopoiesis

祖细胞Hes-1振荡的证实及其对造血分化的影响分析

• 批准号: 23791070
• 负责人: YOKOYAMA Yasuhisa
• 金额: $ 2.66万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23791070/
• 关键词: 血液腫瘍学; Hes-1; 白血病; 発現振動; Hes1; oscillation; leukemia; hematopoiesis

We demonstrated ultradian oscillation of Hes-1 expression in single hematopoietic progenitor cells separated from murine fetal liver. Hematopoietic progenitors introduced with MLL-AF9 fusion gene cause leukemia in in vivo model. When progenitors derived from Hes-1 knockout mice were used, the Hes-1-knockout cells with MLL-AF9 caused earlier death than wild-type cells, which indicated that Hes-1 works as tumor suppressor gene in MLL-AF9 leukemia.

我们从小鼠胎肝分离的单个造血祖细胞中发现了Hes-1表达的超常振荡。MLL-AF9融合基因导入的造血祖细胞在体内引起白血病。当使用Hes-1基因敲除小鼠的祖细胞时，带有MLL-AF9基因的Hes-1基因敲除细胞比野生型细胞更早死亡，这表明Hes-1基因在MLL-AF9白血病中起到了抑瘤基因的作用。

项目成果

Transcription Factor Hes1 Functions As a Tumor Suppressor in MLL-Associated Acute Leukemia

转录因子 Hes1 在 MLL 相关急性白血病中发挥肿瘤抑制作用

• 发表时间: 2012
• 作者: Takayasu Kato;Mamiko Sakata -Yanagimoto;Yasuyuki Miyake;Hidekazu Nishikii;Yasuhisa Yokoyama;and Shigeru Chiba
• 通讯作者: and Shigeru Chiba

Transcription factor Hes1 functions as a tumor suppressor in MLL-associated acute leukemias

转录因子 Hes1 在 MLL 相关急性白血病中充当肿瘤抑制因子

• 发表时间: 2012
• 作者: Takayasu Kato;Mamiko Sakata -Yanagimoto;Yasuyuki Miyake;Hidekazu Nishikii;Yasuhisa Yokoyama;and Shigeru Chiba
• 通讯作者: and Shigeru Chiba