Novel synthetic approach for a positron emitter labeled protein by cell free protein synthesis system

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In this study, we evaluated a novel synthetic approach for a positron emitter labeled protein by cell-free protein synthesis (CFPS) system. Synthesis of ^<11>C-GFP using ^<11>C-Met and CFPS afforded the product within a short time. On the other hand, when using ^<18>F-Pro, the ^<18>F-labeled GFP was produced too slowly to use it practically. We succeeded to prepare^<11>C-labeled scFv antibody to EGFR, which showed specific binding to EGFR-positive tumorcells in vitro and specific uptake by the EGFR-positive tumor in vivo. These results suggest the usefulness of the ^<11>C-labeld protein synthesis by CFPS.