Analysis of novel neoplastic mechanisms of canine mast cell tumors by using a retroviral expression library method.

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Mutations in c-kit gene have been reported to be important as a neoplastic mechanism in canine mast cell tumors (MCTs). In the present study, we investigated the incidence of mutations in c-kit gene in canine MCTs in Japan, and explored novel neoplastic mechanisms by using a retroviral expression library method. We found that mutations in c-kit gene were not detected in more than half of dogs with MCTs. We, next, created retrovirus and tried to establish a retrovirus-mediated gene transfer system to canine bone marrow derived mast cells. However, since transduction efficiency of retrovirus generated in this study was low, we could not identify c-kit gene-independent neoplastic mechanisms of canine MCTs.