Expression of secretory immunoglobulin A in lettuce suitable for the production of therapeutic antibodies in plantfactory.

如今，治疗性抗体作为分子靶向药物被广泛使用。然而，由于成本高且生产可扩展性有限，它们具有局限性。为了降低抗体的生产成本，我们尝试在生菜中表达抗体基因，这种生菜可以在温室中种植，有助于避免对环境的影响。我们制备了对肠出血性大肠杆菌产生的志贺毒素 1 具有特异性的重组 IgA 的 cDNA。我们构建了表达Stx-1特异性IgA的转基因拟南芥和莴苣，并在体外证明了IgA的表达及其毒素中和活性。

Therapeutic antibodies are widely used as molecular-targeted drugs in these days. However, they havelimitations due to the high cost and limited scalability of production. To aim at the lower cost production of antibodies, we tried to express antibody genes in lettuce, which can be cultivated in greenhousethat is useful to avoid environmental impact. We prepared cDNA of recombinant IgA specific for Shiga toxin 1 produced by Enterohaemorrhagic Escherichia coli. We constructed transgenic Arabidopsis thalianaand Lactuca sativaexpressing Stx-1-specific IgA.The expression of IgA and its toxin neutralization activity were demonstrated in vitro.