The development of methods to make changes in the property of induced pluripotent stem cells by the intermittent or sustained hypoxia.

通过间歇性或持续性缺氧改变诱导多能干细胞特性的方法的开发。

• 批准号: 23659109
• 负责人: CHIN Kazuo
• 金额: $ 2.33万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23659109/
• 关键词: intermittent hypoxia,; sustained hypoxia,; pluripotent stem cell,; BEAD-2B cell,; hypoxemia,; hypertension,; lipid metabolism,; sleepiness.; 再生医学; 細胞・組織; 低酸素; 間欠的低酸素; 持続的低酸素

We developed a new hypoxia exposure apparatus for cellular experiments to produce intermittent and sustained hypoxia (IH and SH). We found that IH prevented cellular wound healing in BEADS-2B cells. At appliance, we aimed to develop a new method of altering the natures of induced pluripotent stem cells (iPS cells). However, we have not achieved our primary aim because preliminary studies took more time than planned. Firstly, we needed to develop a new hypoxia exposure apparatus by ourselves because our previous hypoxia exposure apparatus at appliance could not reproduce the stable hypoxic status. Secondly, it was also difficult to acquire sufficient iPS cells because producing iPS cells took much time. We also conducted clinical researches of sleep apnea syndrome, which patients showed intermittent hypoxemia in vivo, to seek for novel biomarkers for IH and investigate the relationships between IH and hypertension, lipid metabolism and sleepiness.

我们研制了一种新的低氧暴露装置，用于细胞实验，以产生间歇性和持续性低氧(IH和SH)。我们发现IH阻止了BEADS-2B细胞的细胞伤口愈合。在应用方面，我们的目标是开发一种新的方法来改变诱导多能干细胞(iPS细胞)的性质。然而，我们并没有达到我们的主要目标，因为初步研究所需的时间比计划的要长。首先，我们需要自行开发一种新的低氧暴露装置，因为我们现有的低氧暴露装置不能再现稳定的低氧状态。其次，也很难获得足够的iPS细胞，因为生产iPS细胞需要很长时间。我们还开展了睡眠呼吸暂停综合征患者体内间歇性低氧血症的临床研究，寻找IH的新生物标志物，并探讨IH与高血压、脂代谢和嗜睡的关系。

项目成果

Kadotani H. Association between sleep apnea, sleep duration, and serum lipid profile in an urban, male, working population in Japan

Kadotani H. 日本城市男性工作人群的睡眠呼吸暂停、睡眠持续时间和血脂状况之间的关联

• 发表时间: 2013
• 期刊: Chest
• 影响因子: 9.6
• 作者: Toyama Y;Chin K;Chihara Y;Takegami M;Takahashi K;Sumi K;Nakamura T;Nakayama-Ashida Y;Minami I;Horita S;Oka Y;Wakamura T;Fukuhara S;Mishima M
• 通讯作者: Mishima M

A urine biomarker for severe obstructive sleep apnoea patients: lipocalin-type prostaglandin D synthase

• DOI: 10.1183/09031936.00133512
• 发表时间: 2013-12-01
• 期刊: EUROPEAN RESPIRATORY JOURNAL
• 影响因子: 24.3
• 作者: Chihara, Yuichi;Chin, Kazuo;Urade, Yoshihiro
• 通讯作者: Urade, Yoshihiro

閉塞性睡眠時無呼吸と脂肪肝含量の関連の検討

阻塞性睡眠呼吸暂停与脂肪肝含量关系的检查

• 发表时间: 2013
• 作者: 玉木英明;阪上洋行;外山善朗
• 通讯作者: 外山善朗

肥満症と睡眠時無呼吸

肥胖和睡眠呼吸暂停

• 发表时间: 2012
• 作者: Katarzyne A. Podyma-Inoue;Miki Yokoyama and Masaki Yanagishita;Yuichiro Arima;八尾寛;陳和夫
• 通讯作者: 陳和夫

Visceral fat accumulation in subjects with non-to-moderate and severe obstructive sleep apnea

患有非中度和重度阻塞性睡眠呼吸暂停的受试者的内脏脂肪堆积

• 发表时间: 2012
• 作者: Chin K;Harada Y;Oga T;Azuma M;Murase K;Toyama Y;Aihara K;Tanizawa K;Chihara Y;Yoshimura C;Hitomi T;Handa T;Mishima M
• 通讯作者: Mishima M