The development of methods to make changes in the property of induced pluripotent stem cells by the intermittent or sustained hypoxia.

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We developed a new hypoxia exposure apparatus for cellular experiments to produce intermittent and sustained hypoxia (IH and SH). We found that IH prevented cellular wound healing in BEADS-2B cells. At appliance, we aimed to develop a new method of altering the natures of induced pluripotent stem cells (iPS cells). However, we have not achieved our primary aim because preliminary studies took more time than planned. Firstly, we needed to develop a new hypoxia exposure apparatus by ourselves because our previous hypoxia exposure apparatus at appliance could not reproduce the stable hypoxic status. Secondly, it was also difficult to acquire sufficient iPS cells because producing iPS cells took much time. We also conducted clinical researches of sleep apnea syndrome, which patients showed intermittent hypoxemia in vivo, to seek for novel biomarkers for IH and investigate the relationships between IH and hypertension, lipid metabolism and sleepiness.