Molecular mechanisms of TGF-b signaling for reprogramming

这是提高诱导多能干细胞（iPS）建立效率的必要条件。细胞的重编程涉及一个中间的间充质-上皮转化（MET）样过程，该过程也受到TGF-ss的调控。本研究试图找出TGF-ss调控MET过程的分子机制。我们使用来自TGF-b受体条件敲除小鼠的小鼠胚胎成纤维细胞（MEF）与CAG-Cre Tg小鼠杂交。然后在多西环素依赖表达系统的控制下，用慢病毒载体编码中山因子，将MEF细胞转化为iPS细胞。这些iPS细胞表现出克隆多样性，需要更长的传代来稳定。

It is required for the improvement and process of establishment efficiency induced pluripotent stem (iPS) cells. Reprogramming of the cells involves an intermediate mesenchymal-epithelial transition (MET)-like process that is also regulated by TGF ss In this study, we have tried to find out the molecularmechanisms in MET process regulated by TGF-ss. We used mouse embryonic fibroblast (MEF) cells from TGF-b receptors conditional knockout mice crossing with CAG-Cre Tg mice. Then we generated iPS cellsfrom the MEF cells by lentivirus vectors which encode Nakayama factors under the control of doxycycline dependent expression system. These iPS cells showed clonal variety and required longer passage for stabilization.