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Establish of iPS by expression of microRNA

通过 microRNA 的表达建立 iPS

基本信息

批准号：
23659148
负责人：
WATANABE Sumiko
金额：
$ 2.41万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Challenging Exploratory Research
财政年份：
2011
资助国家：
日本
起止时间：
2011 至 2012
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23659148/
关键词：
miRNA iPS 分化 MEF RNA microRNA 過剰発現マウスレトロウイルス

项目摘要

miRNA expression pattern of human and mouse iPS was examined by qPCR array followed by bioinformatics, and a list of miRNA, which are expressing highly in iPS, was constructed. Expression vector of these miRNA was made, and they were introduced into MEF, immature iPS, and embryoid body to examine effects of these miRNA for efficiency to establish iPS, immaturity of iPS, and differentiation ability. Several types of expression vector had been constructed, and finally we got a vector expressing miRNA or its antagonized sequence stablyand with high level.

通过qPCR阵列和生物信息学分析了人和小鼠iPS的miRNA表达模式，并构建了在iPS中高表达的miRNA列表。制备这些miRNA的表达载体，并将它们导入MEF、未成熟iPS和胚状体中，以检查这些miRNA对建立iPS的效率、iPS的未成熟性和分化能力的影响。构建了多种表达载体，最终获得了稳定、高效表达miRNA及其拮抗序列的载体。

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

The role of Zic family zinc finger transcription factors in the proliferation and differentiation of retinal progenitor cells.

DOI：
10.1016/j.bbrc.2011.10.007
发表时间：
2011-11
期刊：
Biochemical and biophysical research communications
影响因子：
3.1
作者：
Yui Watabe;Y. Baba;H. Nakauchi;A. Mizota;Sumiko Watanabe
通讯作者：
Yui Watabe;Y. Baba;H. Nakauchi;A. Mizota;Sumiko Watanabe

The early retinal progenitor-expressed gene Sox11 regulates the timing of the differentiation of retinal cells

DOI：
10.1242/dev.090274
发表时间：
2013-02-15
期刊：
DEVELOPMENT
影响因子：
4.6
作者：
Usui, Ayumi;Mochizuki, Yujin;Watanabe, Sumiko
通讯作者：
Watanabe, Sumiko

Serine/threonine kinase, Melk, regulates proliferation and glial differentiation of retinal progenitor cells

DOI：
10.1111/j.1349-7006.2011.02104.x
发表时间：
2012-01
期刊：
Cancer Science
影响因子：
5.7
作者：
Rika Saito;H. Nakauchi;Sumiko Watanabe
通讯作者：
Rika Saito;H. Nakauchi;Sumiko Watanabe

Sox9 is upstream of microRNA-140 in cartilage.

DOI：
10.1007/s12010-011-9404-y
发表时间：
2012-01
期刊：
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
影响因子：
3
作者：
Nakamura, Yukio;He, Xinjun;Kato, Hiroyuki;Wakitani, Shigeyuki;Kobayashi, Tatsuya;Watanabe, Sumiko;Iida, Atsumi;Tahara, Hideaki;Warman, Matthew L.;Watanapokasin, Ramida;Postlethwait, John H.
通讯作者：
Postlethwait, John H.

Dicer Plays Essential Roles for Retinal Development by Regulation of Survival and Differentiation