A role of ADAR1 in induction of somatic hypermutation

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We examined a role of Adenosine deaminase acting on RNA1(ADAR1) in induction of somatic hypermutation (SHM) in germinal center (GC) B cells using ADAR1-transgenic (ADAR1-Tg) and ADAR1-conditional deficient (ADAR1-cKO) mice. After ADAR1-Tg and ADAR1-cKO mice were immunized with NP-CG on Alum, the frequency of SHM in the VH186.2 gene of GC B cells were analyzed. The frequencies in ADAR1-Tg GC B cells and in ADAR1-cKO GC B cells were higher and lower than that in wild type GC B cells, respectively. Thus, ADAR1 plays a role in induction of somatic hypermutations in GC B cells.