Development of targeted cell therapeutic systems based on cell characteristics

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To realized cell therapy, we tried to develop “targeted cell therapeutic systems based on cell characteristics”. We examined the use of multicellular spheroids as a method to maximize the cell survival and functions after in vivo application. Spheroids with controlled size were obtained by using polydimethylsiloxane-based micro-pockets, but cell adhesion to the micro-pocket surface prevented some types of cells from spheroid formation. Coating the micro-pockets with poly(N-isopropylacrylamide) solved this problem, and multicellular spheroids we re successfully prepared using all the cell types used. Then, therapeutic potential of multicellular spheroids was examined in diabetic model mice using insulin-producing NIT -1 cells. NIT-1 spheroids showed prolonged survival of the mice and high activity to reduce blood glucose level compared with NIT -1 cell suspension.