Development of DNA vaccine for Angiotensin II

血管紧张素 II DNA 疫苗的开发

• 批准号: 23659418
• 负责人: KATSUYA Tomohiro
• 金额: $ 2.41万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23659418/
• 关键词: 分子血管病態学; 高血圧; アンジオテンシンII; ワクチン; 血圧

Vaccines have been recently developed to treat various diseases such as cancer, rheumatoid arthritis and Alzheimer’s disease. However, before use in the clinical setting, vaccines targeting self-antigens must be demonstrated to be effective and safe, evoking an adequate humoral immune response from B cells. In this study, we evaluated the efficiency and safety of an Ang II vaccine in mice and rats. Immunization with Ang II conjugated to KLH successfully induced the production of anti-Ang II antibody. In an Ang II-infused model, the non-immunized mice showed high blood pressure (BP), whereas the immunized mice (Ang II-KLH) showed a significant decrease in systolic BP. In terms of DNA vaccine, plasmid vector encoding hepatitis B core (HBc)- Ang II fusion protein was injected into spontaneously hypertensive rats (SHR). Anti-Ang II antibody was successfully produced in HBc-Ang II group, and antibody titers were sustained for at least 6 months. Systolic BP was consistently lower in HBc-Ang II group after immunization.

最近已经开发出疫苗来治疗各种疾病，例如癌症、类风湿性关节炎和阿尔茨海默病。然而，在临床使用之前，靶向自身抗原的疫苗必须被证明是有效和安全的，从B细胞中引起足够的体液免疫应答。在这项研究中，我们评估了血管紧张素II疫苗在小鼠和大鼠中的有效性和安全性。用与KLH缀合的Ang II免疫成功诱导抗Ang II抗体的产生。在血管紧张素II输注模型中，未免疫小鼠显示高血压（BP），而免疫小鼠（血管紧张素II-KLH）显示收缩压显着下降。在DNA疫苗方面，将编码乙型肝炎B核心蛋白（HBc）-血管紧张素II（Ang II）融合蛋白的质粒载体注射到自发性高血压大鼠（SHR）体内。HBc-Ang Ⅱ组成功产生抗Ang Ⅱ抗体，抗体滴度持续至少6个月。免疫后HBc-Ang II组的收缩压持续降低。

项目成果

Development of DNA vaccine for high blood pressure -Immunotherapy for angiotensin II -

高血压DNA疫苗的开发-血管紧张素II的免疫疗法-

• 发表时间: 2011
• 作者: Sugimura K;Fukumoto Y;Satoh K;Nochioka K;Miura Y;Aoki T;Tatebe S;Miyamichi-Yamamoto S;Shimokawa H;中神啓徳
• 通讯作者: 中神啓徳

Decrease in blood pressure and regression of cardiovascular complications by angiotensin II vaccine in mice.

• DOI: 10.1371/journal.pone.0060493
• 发表时间: 2013
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Nakagami F;Koriyama H;Nakagami H;Osako MK;Shimamura M;Kyutoku M;Miyake T;Katsuya T;Rakugi H;Morishita R
• 通讯作者: Morishita R

アンジオテンシンIIを標的とした高血圧ワクチンの基礎研究

血管紧张素II靶向高血压疫苗基础研究

• 发表时间: 2012
• 作者: 升本英利;清水達也;池田義;岡野光夫;坂田隆造;山下潤;中神啓徳
• 通讯作者: 中神啓徳

高血圧自然発症ラット(SHR)モデルにおけるアンジオテンシン II ワクチン治療法の開発

自发性高血压大鼠（SHR）模型血管紧张素II疫苗疗法的开发

• 发表时间: 2011
• 作者: 森下竜一;中神啓徳
• 通讯作者: 中神啓徳