Establishment of TaqMan PCR method for the genetic analysis of cardiovascular disease

心血管疾病基因分析TaqMan PCR方法的建立

• 批准号: 12557063
• 负责人: KATSUYA Tomohiro
• 金额: $ 4.1万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12557063/
• 关键词: TaqMan PCR; cost performance; genetics; SNPs; cardiovascular disease; hypertension; DNA array; genetic susceptibility; SNPs; 対立遺伝子; TaqMan; プローブ; 連鎖不平衡

DNA array technique is sophisticated approach to examine huge number of genes involved in the disease susceptibility. However, it is not appropriate to examine several candidate gene polymorphisms in the large number of subjects. TaqMan chemistry was established to carry out quantitative RT-PCR and applied the technique of the genotyping of single nucleotide polymorphism. We made the best use of inventiveness, resulted that new sophisticated genotyping system, TaqMan PCR method, was achieved with high success rate and high cost performance. Especially, we succeeded to develop the method which able to determine the insertion/deletion polymorphism of angiotensin converting enzyme gene using TaqMan PCR method (Patent Pending: Patent #2004-119417, Identification#504148642). TaqMan PCR method also allowed us to carry out many association studies to examine the correlation between SNPs and disease (cardiovascular disease or hypertension) susceptibility and we published 39 papers of cardiovascular genetics showing positive or negative associations in the disease predisposition. We believe that TaqMan PCR method is useful for the realization of tailored medicine.

DNA微阵列技术是一种检测与疾病易感性相关的大量基因的先进方法。然而，在大量的受试者中检查几个候选基因多态性是不合适的。建立TaqMan化学定量RT-PCR方法，应用单核苷酸多态性基因分型技术。我们充分发挥了创造性，实现了一种新的高成功率和高性价比的基因分型系统-TaqMan PCR方法。特别地，我们成功地开发了能够使用TaqMan PCR方法确定血管紧张素转换酶基因的插入/缺失多态性的方法（专利申请：专利#2004-119417，鉴定#504148642）。TaqMan PCR方法也使我们能够进行许多关联研究，以检查SNPs与疾病（心血管疾病或高血压）易感性之间的相关性，我们发表了39篇心血管遗传学论文，显示疾病易感性的正或负关联。我们认为TaqMan PCR方法有助于实现个体化药物治疗。

项目成果

Motone M, Katsuya T, et al.: "Association between hepatocyte growth factor gene polymorphism and essential hypertension"Hypertens Res. 27(4). 247-251 (2004)

Motone M、Katsuya T 等人：“肝细胞生长因子基因多态性与原发性高血压之间的关联”Hypertens Res。

Matsubara M, Katsuya T, et al.: "Angiotensin-converting enzyme I/D polymorphism and hypertension : the Ohasama study"J Hypertens. 20(6). 1121-1126 (2002)

Matsubara M、Katsuya T 等人：“血管紧张素转换酶 I/D 多态性和高血压：Ohasama 研究”J Hypertens。

Inamoto N, Katsuya T, et al.: "Association of methylenetetrahydrofolate reductase gene polymorphism with carotid atherosclerosis depending on smoking status in a Japanese general population."Stroke. 34(7). 1628-1633 (2003)

Inamoto N、Katsuya T 等人：“亚甲基四氢叶酸还原酶基因多态性与颈动脉粥样硬化的关联取决于日本普通人群的吸烟状况。”中风。

Kikuya M, Katsuya T, et al.: "A/C1166 gene polymorphism of the angiotensin II type 1 receptor (AT1) and ambulatory blood pressure : the Ohasama Study."Hypertens Res. 26(2). 141-145 (2003)

Kikuya M、Katsuya T 等人：“血管紧张素 II 1 型受体 (AT1) 的 A/C1166 基因多态性与动态血压：Ohasama 研究。”Hypertens Res。

Matsubara M, Katsuya T, et al.: "T+31C polymorphism (M235T) of the angiotensinogen gene and home blood pressure in the Japanese general population : the Ohasama Study"Hypertens Res. 26(1). 47-52 (2003)

Matsubara M、Katsuya T 等人：“血管紧张素原基因的 T 31C 多态性 (M235T) 与日本一般人群的家庭血压：Ohasama 研究”Hypertens Res。