Elucidation of molecular mechanisms involved in the immunomodulatory function of newly developed adipose tissue derived mesenchymal stem cells

阐明新开发的脂肪组织来源的间充质干细胞的免疫调节功能的分子机制

• 批准号: 23659443
• 负责人: MATSUO Seiichi
• 金额: $ 2.41万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23659443/
• 关键词: 幹細胞; 再生医療; 免疫抑制; 脂肪由来間葉系幹細胞; 強皮症; マクロファージ; メタボローム解析

We have proven that low serum cultured adipose derived mesenchymal stem cells (LASC) suppress T cell proliferation and induce polarization of macrophages into immunomodulatory M2 type, and that these effects of LASC were much stronger than high serum cultured adipose derived mesenchymal stem cells (HASC) or bone marrow derived mesenchymal stem cells (BM-MSC). Inthis study, we studied the mechanisms involved in the immunosuppressive functionof LASC by comparing with HASC or BM-MSC. We found by neutralization experiment and stimulation experiment that LASC derived PGE2 and IL-6 are necessary for the polarization of M2 macrophages, and that PGE2 function via EP4 receptor.

低血清培养的脂肪间充质干细胞（adipose derived mesenchymal stem cells，LASC）可抑制T细胞增殖，诱导巨噬细胞向M2型分化，其作用强于高血清培养的脂肪间充质干细胞（adipose derived mesenchymal stem cells，HASC）和骨髓间充质干细胞（bone marrow derived mesenchymal stem cells，BM-MSC）。本研究通过与HASC和BM-MSC的比较，探讨LASC免疫抑制作用的机制。我们通过中和实验和刺激实验发现，LASC来源的PGE 2和IL-6对于M2巨噬细胞的极化是必需的，并且PGE 2通过EP 4受体发挥作用。

项目成果

Clinical findings on ANCA-associated renal vasculitis from the Japan RPGN registry obtained via a questionnaire survey.

日本 RPGN 登记处通过问卷调查获得的 ANCA 相关肾血管炎临床结果。

• DOI: 10.1007/s10157-012-0740-1
• 发表时间: 2013
• 期刊: Clin Exp Nephrol
• 影响因子: 2.3
• 作者: Yamagata K;Usui J;Sugiyama H;Nitta K;Wada T;Muso E;Arimura Y;Koyama A;Makino H;Matsuo S.
• 通讯作者: Matsuo S.

腹膜透析合同レジストリーにおける適正透析、腹膜機能に関する検討

在腹膜透析联合登记处检查适当的透析和腹膜功能

• 发表时间: 2011
• 作者: Kataoka K;et al;杉山 斉
• 通讯作者: 杉山 斉

CE-MS based time-course metabolic profiling in plasma and dialysate with hemodialysis

基于 CE-MS 的血液透析血浆和透析液的时程代谢分析

• 发表时间: 2011
• 作者: Yonekawa T;Matsushita T;Minohara M;Isobe N;Masaki K;Yoshimura S;Nishimura S;Kira J:;古橋和拡 清水明日香 阿部智子 金恒秀 勝野敬之 尾崎武徳 ,坪井直毅 丸山彰一 松尾清一;Kira J et al;Hiroshi Kato;Kira J et al;Shin’ichi Akiyama
• 通讯作者: Shin’ichi Akiyama

ANCA-associated systemic vasculitis in Japan: clinical features and prognostic changes

• DOI: 10.1007/s10157-012-0598-2
• 发表时间: 2012-08-01
• 期刊: CLINICAL AND EXPERIMENTAL NEPHROLOGY
• 影响因子: 2.3
• 作者: Yamagata, Kunihiro;Usui, Joichi;Matsuo, Seiichi
• 通讯作者: Matsuo, Seiichi

慢性血液透析患者における心血管、総死亡率の予後因子ＧＮＲＩ

GNRI 是慢性血液透析患者心血管和全因死亡率的预后因素。

• 作者: Toshihiro Shirakawa;Takashi Horiyama,and Ryuhei Uehara;安田香
• 通讯作者: 安田香