Elucidation of the target genes for the treatment of lipid disorder by gene-trap method in human haploid cells
Elucidation of the target genes for the treatment of lipid disorder by gene-trap method in human haploid cells
批准号:
23659469
负责人:
ONO Koh
金额:
$2.33万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Challenging Exploratory Research
财政年份:
2011
资助国家:
日本
项目状态:
已结题
起止时间:
2011 至 2012
中文摘要
我们建立了基因捕获法并将其应用于脂肪细胞的分化。然而,哺乳动物细胞是二倍体,并且只有具有单倍体不足的基因可以通过该方法获得。这次我们使用了人类单倍体细胞系P1-55细胞,并能够通过基因捕获方法筛选所有基因。
英文摘要
We have developed gene-trap method and applied it to adipocyte differentiation. However, mammalian cells are diploid and only such genes which have haploid insufficiency can be obtained by this method. This time we utilized P1-55 cells, which is a human haploid cell line, and enabled to screen all genes by gene-trap method.
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MicroRNA 26b encoded by the intron of SCP1 has an antagonistic on its host gene and cardiac hypertrophy
SCP1内含子编码的MicroRNA 26b对其宿主基因具有拮抗作用并导致心脏肥大
DOI:
--
发表时间:
2012
期刊:
影响因子:
--
作者:
[Sowa N, Ono K, Horie T, Hasegawa K, Kita T, Kimuta T]
通讯作者:
Kimuta T
Symposium 6 (Insulin resistance) MicroRNA regulation of lipid homeostasis
研讨会6(胰岛素抵抗)脂质稳态的MicroRNA调节
DOI:
--
发表时间:
2012
期刊:
影响因子:
--
作者:
[Koichiro Kuwahara, Kazuwa Nakao, Akazawa H, Ono K]
通讯作者:
Ono K
MicroRNA-33 encoded by an intron of Srebp2 regulates HDL in vivo
Srebp2 内含子编码的 MicroRNA-33 在体内调节 HDL
DOI:
--
发表时间:
2010
期刊:
Proc Natl Acad Sci U S A.
影响因子:
--
作者:
[Horie T, Ono K, Horiguchi M, Nishi H, Nakamura T, Nagao K, Kinoshita M, Kuwabara Y, Marusawa H, Iwanaga Y, Hasegawa K, Yokode M, Kimura T, Kita T.]
通讯作者:
Kita T.
DOI:
10.1002/jcb.24222
发表时间:
2012-11-01
期刊:
JOURNAL OF CELLULAR BIOCHEMISTRY
影响因子:
4
作者:
[Sowa, Naoya, Horie, Takahiro, Ono, Koh]
通讯作者:
Ono, Koh
Increased microRNA-1 and microRNA-133a Levels in Serum of Patients with Cardiovascular Disease Indicate the Existence of Myocardial Damage
心血管疾病患者血清中microRNA-1和microRNA-133a水平升高提示心肌损伤的存在
DOI:
--
发表时间:
2011
期刊:
Circulation: Cardiovascular Genetics
影响因子:
--
作者:
[Kuwabara Y, Ono K, Horie T, Nishi H, Nagao K, Kinoshita M, Watanabe S, Baba O, Kojima Y, Shizuta S, Imai M, Tamura T, Kita T, Kimura T.]
通讯作者:
Kimura T.
共 36 条
Role of microRNAs on metabolic control in the heart
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批准号:20590864
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$3.0万
-
财政年份:2008
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负责人:ONO Koh
-
依托单位:
Establishment of the therapeutic strategy for the cardiovascular complications in Metabolic syndrome by gene trap method
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批准号:18590828
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$2.57万
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财政年份:2006
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负责人:ONO Koh
-
依托单位:
海外基金