Development of a novel cancer therapy using soluble T-cell receptor

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In this study, we attempted to establish a novel immunotherapyfor cancer using tumor antigen-specific soluble T-cell receptor (TCR). The data obtained from the series of experiments are follows.1) WT1, hTERT, and Aurora-A kinase-derived peptide-specific cytotoxic T cell (CTL) clones were established.2) TCR genes were isolated from these CTL clones.3) A novel retrovirus vector expressing TCR genes and inhibiting expression of endogenous TCR has been developed.4) WT1-specific hTERT-specific, and Aurora-A kinase-specific TCR gene-transduced T cells showed antigen-specific cytotoxicity and exerted anti-leukemia effects in vitro and in vivo.Now, we areattempting to produce WT1-specific soluble TCR molecules.